Price range: $268.00 through $328.00
Background:
This gene is encodes a mitochondrial protein that contains a BH3 domain and acts as a pro-apoptotic factor. The encoded protein interacts with anti-apoptotic proteins, including the E1B 19 kDa protein and Bcl2. This gene is silenced in tumors by DNA methylation. [provided by RefSeq, Dec 2014],
| Size | 50μL/100μL |
| Target | BNIP-3 |
| Reactivity | Human, Mouse |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 22kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC: 1:100-300; ELISA 1:20000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from the Internal region of human BNIP3. AA range:71-120 |
| Specificity | BNIP-3 Polyclonal Antibody detects endogenous levels of BNIP-3 protein. |
| Gene Name | BNIP3 |
| Protein Name | BCL2/adenovirus E1B 19 kDa protein-interacting protein 3 |
| Other Name | BNIP3; / NIP3; / BCL2/adenovirus E1B 19 kDa protein-interacting protein 3 |
| Species | Human |
| Gene ID | 664 |
| Uniprot | Q12983, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | O55003 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Mitochondrion. Mitochondrion outer membrane; Single-pass membrane protein. Coexpression with the EIB 19-kDa protein results in a shift in NIP3 localization pattern to the nuclear envelope. Colocalizes with ACAA2 in the mitochondria. Colocalizes with SPATA18 at the mitochondrion outer membrane. |
| Tissue Expression | B-cell,Brain,Cerebellum,Kidney,Testis, |
| Signaling_pathway | Cellular Processes >> Transport and catabolism >> Autophagy - animal;Cellular Processes >> Transport and catabolism >> Mitophagy - animal;Environmental Information Processing >> Signal transduction >> FoxO signaling pathway |
| Research Areas | >>FoxO signaling pathway; / >>Mitophagy - animal; / >>Autophagy - animal; / >>Shigellosis; / >>Legionellosis |
| Function | Function:Apoptosis-inducing protein that, which can overcome BCL2 suppression. May play a role in repartitioning calcium between the two major intracellular calcium stores in association with BCL2.,similarity:Belongs to the NIP3 family.,subcellular location:Coexpression with the EIB 19-kDa protein results in a shift in NIP3 localization pattern to the nuclear envelope.,subunit:Homodimer. Binds to BCL2. Interacts with BNIP3L. Also can interact with adenovirus E1B 19 kDa protein or Epstein-Barr virus BHRF1., |
