Price range: $268.00 through $328.00
Background:
BOB.1 is a transcription activating factor necessary for the formation of follicular germinal center and the production of immunoglobulin. It works together with Oct-2 and is highly expressed in granulocytes of spleen and peripheral blood. It is mainly used for the typing of Hodgkin’s lymphoma, mainly nodular lymphocytes. Hodgkin’s lymphoma is positive and classical Hodgkin’s lymphoma is negative.
| Target | BOB.1 |
| Reactivity | Human, Mouse |
| Applications | IHC, WB, IF, ELISA |
| MW(Calculated) | 27kD |
| MW(Observed) | 35kD |
| Host Species | Mouse |
| Isotype | IgG2a,Kappa |
| Conjugate/Modification | Unmodified |
| Modification | |
| Recommended Dilution Ratio | IHC 1:200-1000; WB 1:500-2000; IF 1:100-500; ELISA 1:1000-5000 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#YS0004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Source | |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein G affinity chromatography. |
| Purity | |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | ABT-BOB1 |
| Immunogen | Synthesized peptide derived from human BOB.1 AA range: 150-256 |
| Sequence | |
| Specificity | The antibody can specifically recognize human BOB.1 protein. |
| Gene Name | POU2AF1 OBF1 |
| Protein Name | POU domain class 2-associating factor 1 (B-cell-specific coactivator OBF-1) (BOB-1) (OCA-B) (OCT-binding factor 1) |
| Other Name | B cell Oct binding protein 1; / B cell specific coactivator OBF 1; / B cell specific coactivator OBF1; / B-cell-specific coactivator OBF-1; / BOB 1; / BOB-1; / OBF 1; / OBF1; / OBF1_HUMAN; / OCA B; / OCA-B; / OCAB; / OCT binding factor 1; / OCT-binding factor 1; / POU class 2 associating factor 1; / POU domain class 2 associating factor 1; / POU domain class 2-associating factor 1; / Pou2af1 |
| Species | Human |
| Gene ID-1 | 5450 |
| Uniprot | Q16633, |
| Species.1 | Mouse |
| Gene ID-2 | 18985 |
| Uniprot.1 | Q64693 |
| Species.2 | |
| Gene ID-3 | |
| Uniprot.2 | |
| Organism-4 | |
| Gene ID-4 | |
| SwissProt-4 | |
| Background | BOB.1 is a transcription activating factor necessary for the formation of follicular germinal center and the production of immunoglobulin. It works together with Oct-2 and is highly expressed in granulocytes of spleen and peripheral blood. It is mainly used for the typing of Hodgkin's lymphoma, mainly nodular lymphocytes. Hodgkin's lymphoma is positive and classical Hodgkin's lymphoma is negative. |
| Cellular Localization | Nuclear, Cytoplasmic |
| Tissue Expression | B-cell specific (PubMed:7779176, PubMed:7859290). Detected in mainly in spleen, but also in thymus, periphral blood leukocyte and small intestine (PubMed:7779176, PubMed:7859290). |
| Signaling_pathway | |
| Research Areas | |
| Function | Disease:A chromosomal aberration involving POU2AF1/OBF1 may be a cause of a form of B-cell leukemia. Translocation t(3; 11)(q27; q23) with BCL6.,Function:Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers.,PTM:Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.,similarity:Belongs to the POU2AF1 family.,tissue specificity:B-cell specific., |
| RRID |
