Price range: $268.00 through $328.00
Background:
cell division cycle associated 8(CDCA8) Homo sapiens This gene encodes a component of the chromosomal passenger complex. This complex is an essential regulator of mitosis and cell division. This protein is cell-cycle regulated and is required for chromatin-induced microtubule stabilization and spindle formation. Alternate splicing results in multiple transcript variants. Pseudgenes of this gene are found on chromosomes 7, 8 and 16. [provided by RefSeq, Apr 2013],
| Size | 50μL/100μL |
| Target | BOREA |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, ELISA |
| MW(Calculated) | |
| MW(Observed) | 30kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-2000; ELISA 1:5000-20000 |
| Formulation | Liquid in PBS containing 50% glycerol,0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | Synthesized peptide derived from part region of human protein |
| Specificity | BOREA Polyclonal Antibody detects endogenous levels of protein. |
| Gene Name | CDCA8 PESCRG3 |
| Protein Name | Borealin (Cell division cycle-associated protein 8) (Dasra-B) (hDasra-B) (Pluripotent embryonic stem cell-related gene 3 protein) |
| Other Name | |
| Species | Human |
| Gene ID | 55143 |
| Uniprot | Q53HL2, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | Q8BHX3, |
| Species.2 | Rat |
| Gene ID.2 | |
| Uniprot.2 | Q6AXW0 |
| Cellular Localization | Nucleus, nucleolus . Cytoplasm . Cytoplasm, cytoskeleton, spindle . Chromosome, centromere . Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalizes with SENP3 in the nucleolus in interphase cells. . |
| Tissue Expression | Colon,Coronary arterial endothelium,Embryo,Epithelium,Kidney,Lung, |
| Signaling_pathway | |
| Research Areas | |
| Function | developmental stage:Cell-cycle regulated. Increases during G2/M phase and then reduces after exit from M phase.,Function:Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. In the complex, it may be required to direct the CPC to centromeric DNA.,miscellaneous:Cells lacking CDCA8 display a slight decrease in histone H3 'Ser-10' phosphorylation, suggesting that the CPC complex mediates phosphorylation of 'Ser-10' of histone H3.,similarity:Belongs to the borealin family.,subcellular location:Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis.,subunit:May form homooligomers. Component of the CPC at least composed of BIRC5/survivin, CDCA8/borealin, INCENP and AURKB/Aurora-B., |
