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Home/Reagents/Antibody/Primary antibody/Polyclonal Antibody/Rabbit pAb/BRE Polyclonal Antibody
SKU: WP11304 Categories: ,

BRE Polyclonal Antibody

Ucallm_WP11304-50

Price range: $268.00 through $328.00

SKU: WP11304-100
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Product details

Background:

This gene encodes an anti-apoptotic, death receptor-associated protein that interacts with tumor necrosis factor-receptor-1. The encoded protein acts as an adapter in several protein complexes, including the BRCA1-A complex and the BRISC complex. The BRCA1-A complex possesses ubiquitinase activity and targets sites of double strand DNA breaks, while the BRISC complex exhibits deubiquitinase activity and is involved in mitotic spindle assembly. This gene is upregulated in several types of cancer. [provided by RefSeq, Jun 2016],

Specifications

Size50μL/100μL
TargetBRE
ReactivityHuman, Mouse, Rat
ApplicationsWB, ELISA
MW(Calculated)
MW(Observed)47kD
Host SpeciesRabbit
IsotypeIgG
Conjugate/ModificationUnmodified
Recommended Dilution RatioWB 1:500-1:2000; ELISA 1:10000; Not yet tested in other applications.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration1 mg/ml
ClonalityPolyclonal
Clone Number
ImmunogenThe antiserum was produced against synthesized peptide derived from human BRE. AA range:325-374
SpecificityBRE Polyclonal Antibody detects endogenous levels of BRE protein.
Gene NameBRE
Protein NameBRCA1-A complex subunit BRE
Other NameBRE; / BRCC45; / BRCA1-A complex subunit BRE; / BRCA1/BRCA2-containing complex subunit 45; / Brain and reproductive organ-expressed protein
SpeciesHuman
Gene ID9577
UniprotQ9NXR7,
Species.1Mouse
Gene ID.1107976
Uniprot.1Q8K3W0,
Species.2Rat
Gene ID.2362704
Uniprot.2Q6P7Q1
Cellular LocalizationCytoplasm . Nucleus . Localizes at sites of DNA damage at double-strand breaks (DSBs). .
Tissue ExpressionExpressed in all cell lines examined. Highly expressed in placenta.
Signaling_pathway
Research Areas>>Homologous recombination
FunctionAlternative products:Additional isoforms may exist,Domain:Contains 2 ubiquitin-conjugating enzyme family-like (UEV-like) regions. These regions lack the critical Cys residues required for ubiquitination but retain the ability to bind ubiquitin.,Function:Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. In the BRCA1-A complex, it acts as an adapter that bridges the interaction between MERIT40/NBA1 and the rest of the complex, thereby being required for the complex integrity and modulating the E3 ubiquitin ligase activity of the BRCA1-BARD1 heterodimer. Probably also plays a role as a component of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin. May play a role in homeostasis or cellular differentiation in cells of neural, epithelial and germline origins. May also act as a death receptor-associated anti-apoptotic protein, which inhibits the mitochondrial apoptotic pathway. May regulate TNF-alpha signaling through its interactions with TNFRSF1A; however these effects may be indirect.,induction:Down-regulated by DNA-damaging agents in fibroblasts, by retinoic acid in brain glioma U-251 and promyelocytic HL-60 cell lines, and by LPS in peripheral blood mononuclear cells (PBMC).,similarity:Belongs to the BRE family.,subcellular location:Localizes at sites of DNA damage at double-strand breaks (DSBs).,subunit:Component of the BRCA1-A complex, at least composed of the BRCA1, BARD1, UIMC1/RAP80, FAM175A/Abraxas, BRCC3/BRCC36, BRE/BRCC45 and MERIT40/NBA1. In the BRCA1-A complex, interacts directly with FAM175A/Abraxas, BRCC3/BRCC36 and MERIT40/NBA1. Binds polyubiquitin. Component of the BRISC complex, at least composed of the FAM175B/ABRO1, BRCC3/BRCC36, BRE/BRCC45 and MERIT40/NBA1. Component of the BRCA1/BRCA2 containing complex (BRCC), which also contains BRCA1, BRCA2, BARD1, BRCC3/BRCC36 and RAD51. BRCC is a ubiquitin E3 ligase complex that enhances cellular survival following DNA damage. May interact with FAS and TNFRSF1A.,tissue specificity:Expressed in all cell lines examined. Highly expressed in placenta.,
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