Price range: $268.00 through $328.00
Background:
Caldesmon is a binding protein of smooth muscle actin and calmodulin. It is a cytoskeleton related protein with the function of regulating smooth muscle contraction. In clinical diagnosis, it is used to label smooth muscle cells and cells with smooth muscle differentiation, identify myofibroblastoma from true leiomyoma, and combine CD10 to identify endometrial stromal sarcoma and uterine smooth muscle tumor.
| Size | 50μL/100μL |
| Target | Caldesmon |
| Reactivity | Human |
| Applications | IHC, WB, IF, ELISA |
| MW(Calculated) | 93kD |
| MW(Observed) | 93kD |
| Host Species | Mouse |
| Isotype | IgG2a,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1000; WB 1:500-2000; IF 1:100-500; ELISA 1:1000-5000 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein G affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RMM125 |
| Immunogen | Synthesized peptide derived from human Caldesmon pan AA range: 100-200 |
| Specificity | The antibody can recognize human h-caldesmon and l-caldesmon protein. In western blotting , the antibody labels a 93 kDa band. |
| Gene Name | CALD1 CAD CDM |
| Protein Name | Caldesmon |
| Other Name | CALD1; / CAD; / CDM; / Caldesmon; / CDM |
| Species | Human |
| Gene ID | 800 |
| Uniprot | Q05682 |
| Species.1 | |
| Gene ID.1 | |
| Uniprot.1 | |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Cytoplasmic |
| Tissue Expression | High-molecular-weight caldesmon (isoform 1) is predominantly expressed in smooth muscles, whereas low-molecular-weight caldesmon (isoforms 2, 3, 4 and 5) are widely distributed in non-muscle tissues and cells. Not expressed in skeletal muscle or heart. |
| Signaling_pathway | Organismal Systems >> Circulatory system >> Vascular smooth muscle contraction |
| Research Areas | >>Vascular smooth muscle contraction |
| Function | Domain:The N-terminal part seems to be a myosin/calmodulin-binding domain, and the C-terminal a tropomyosin/actin/calmodulin-binding domain. These two domains are separated by a central helical region in the smooth-muscle form.,Function:Actin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). Stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, inhibits the actomyosin ATPase by binding to F-actin. This inhibition is attenuated by calcium-calmodulin and is potentiated by tropomyosin. Interacts with actin, myosin, two molecules of tropomyosin and with calmodulin. Also play an essential role during cellular mitosis and receptor capping.,PTM:In non-muscle cells, phosphorylation by CDC2 during mitosis causes caldesmon to dissociate from microfilaments. Phosphorylation reduces caldesmon binding to actin, myosin, and calmodulin as well as its inhibition of actomyosin ATPase activity. Phosphorylation also occurs in both quiescent and dividing smooth muscle cells with similar effects on the interaction with actin and calmodulin and on microfilaments reorganization.,similarity:Belongs to the caldesmon family.,subcellular location:On thin filaments in smooth muscle and on stress fibers in fibroblasts (nonmuscle).,tissue specificity:High-molecular-weight caldesmon (isoform 1) is predominantly expressed in smooth muscles, whereas low-molecular-weight caldesmon (isoforms 2, 3, 4 and 5) are widely distributed in non-muscle tissues and cells. Not expressed in skeletal muscle or heart., |
