Caspase-10 p23/17 (Cleaved-D415) rabbit pAb
Ucallm_WP0106-50
Price range: $268.00 through $328.00
Product details
Background:
This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 3 and 7, and the protein itself is processed by caspase 8. Mutations in this gene are associated with type IIA autoimmune lymphoproliferative syndrome, non-Hodgkin lymphoma and gastric cancer. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Apr 2011],
Specifications
| Size | 50μL/100μL |
| Target | Caspase-10 |
| Reactivity | Human |
| Applications | WB, ELISA |
| MW(Calculated) | |
| MW(Observed) | 23kD/17kD 55kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:1000-2000; ELISA 1:5000-20000 |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | Synthesized peptide derived from human Caspase-10 p23/17 (Cleaved-D415) |
| Specificity | This antibody detects endogenous levels of Human Caspase-10 p23/17 (Cleaved-D415, protein was cleaved amino acid sequence between 415-416) |
| Gene Name | CASP10 MCH4 |
| Protein Name | Caspase10 |
| Other Name | Caspase-10; / CASP-10; / Apoptotic protease Mch-4; / FAS-associated death domain protein interleukin-1B-converting enzyme 2; / FLICE2; / ICE-like apoptotic protease 4; / [Cleaved into: Caspase-10 subunit p23/17; / Caspase-10 subunit p12] |
| Species | Human |
| Gene ID | 843 |
| Uniprot | Q92851 |
| Species.1 | |
| Gene ID.1 | |
| Uniprot.1 | |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | |
| Tissue Expression | Detectable in most tissues. Lowest expression is seen in brain, kidney, prostate, testis and colon. |
| Signaling_pathway | Cellular Processes >> Cell growth and death >> Apoptosis;Organismal Systems >> Immune system >> RIG-I-like receptor signaling pathway;Environmental Information Processing >> Signal transduction >> TNF signaling pathway |
| Research Areas | >>Apoptosis; / >>RIG-I-like receptor signaling pathway; / >>TNF signaling pathway; / >>Tuberculosis; / >>Hepatitis B |
| Function | Catalytic activity:Strict requirement for Asp at position P1 and has a preferred cleavage sequence of Leu-Gln-Thr-Asp-|-Gly.,Disease:Defects in CASP10 are a cause of familial non-Hodgkin lymphoma (NHL) [MIM:605027]. NHL is a cancer that starts in cells of the lymph system, which is part of the body's immune system. NHLs can occur at any age and are often marked by enlarged lymph nodes, fever and weight loss.,Disease:Defects in CASP10 are a cause of gastric cancers [MIM:137215].,Disease:Defects in CASP10 are the cause of autoimmune lymphoproliferative syndrome type 2A (ALPS2A) [MIM:603909]. ALPS2 is characterized by abnormal lymphocyte and dendritic cell homeostasis and immune regulatory defects.,Function:Involved in the activation cascade of caspases responsible for apoptosis execution. Recruited to both Fas- and TNFR-1 receptors in a FADD dependent manner. May participate in the granzyme B apoptotic pathways. Cleaves and activates caspase-3, -4, -6, -7, -8, and -9. Hydrolyzes the small- molecule substrates, Tyr-Val-Ala-Asp-|-AMC and Asp-Glu-Val-Asp-|-AMC.,Function:Isoform C is proteolytically inactive.,online information:CASP10 mutation db,online information:Caspase-10 mutations causing ALPS type II,PTM:Cleavage by granzyme B and autocatalytic activity generate the two active subunits.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the peptidase C14A family.,similarity:Contains 2 DED (death effector) domains.,subunit:Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 23/17 kDa (p23/17) (depending on the splicing events) and a 12 kDa (p12) subunit (By similarity). Self-associates. Interacts with FADD and CASP8. Found in a Fas signaling complex consisting of FAS, FADD, CASP8 and CASP10.,tissue specificity:Detectable in most tissues. Lowest expression is seen in brain, kidney, prostate, testis and colon., |
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