SKU: WP11450 Categories: ,

CBP20 Polyclonal Antibody

Price range: $268.00 through $328.00

SKU: WP11450-100
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Product details

Background:

The product of this gene is a component of the nuclear cap-binding protein complex (CBC), which binds to the monomethylated 5' cap of nascent pre-mRNA in the nucleoplasm. The encoded protein has an RNP domain commonly found in RNA binding proteins, and contains the cap-binding activity. The CBC promotes pre-mRNA splicing, 3'-end processing, RNA nuclear export, and nonsense-mediated mRNA decay. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],

Specifications

Size50μL/100μL
TargetCBP20
ReactivityHuman, Mouse
ApplicationsWB, IHC, IF, ELISA
MW(Calculated)
MW(Observed)18kD
Host SpeciesRabbit
IsotypeIgG
Conjugate/ModificationUnmodified
Recommended Dilution RatioWB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:5000; Not yet tested in other applications. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration1 mg/ml
ClonalityPolyclonal
Clone Number
ImmunogenThe antiserum was produced against synthesized peptide derived from human NCBP2. AA range:10-59
SpecificityCBP20 Polyclonal Antibody detects endogenous levels of CBP20 protein.
Gene NameNCBP2
Protein NameNuclear cap-binding protein subunit 2
Other NameNCBP2; / CBP20; / PIG55; / Nuclear cap-binding protein subunit 2; / 20 kDa nuclear cap-binding protein; / Cell proliferation-inducing gene 55 protein; / NCBP 20 kDa subunit; / CBP20; / NCBP-interacting protein 1; / NIP1
SpeciesHuman
Gene ID22916
UniprotP52298,
Species.1Mouse
Gene ID.168092
Uniprot.1Q9CQ49
Species.2
Gene ID.2
Uniprot.2
Cellular LocalizationNucleus . Cytoplasm .
Tissue ExpressionCervix,Cervix carcinoma,Human small intestine,
Signaling_pathwayGenetic Information Processing >> Translation >> Nucleocytoplasmic transport
Research Areas>>Nucleocytoplasmic transport; / >>mRNA surveillance pathway; / >>Spliceosome
FunctionFunction:Involved in mediating U snRNA export from the nucleus. Binds to 5' capped mRNA.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the RRM NCBP2 family.,similarity:Contains 1 RRM (RNA recognition motif) domain.,subunit:Found in a U snRNA export complex with RNUXA/PHAX, NCBP1, NCBP2, RAN, XPO1 and m7G-capped RNA (By similarity). Heterodimer with NCBP1. Is part of the exon junction complex (EJC) containing NCBP1, NCBP2, RNPS1, RBM8A, SRRM1, NXF1, UPF3B, UPF2, THOC4 and/or REFBP2.,
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