Price range: $268.00 through $328.00
Background:
This gene encodes a cell surface receptor expressed on natural killer (NK) cells (and some T cells) that mediate non-major histocompatibility complex (MHC) restricted killing. The interaction between NK-cell and target cells via this receptor is thought to modulate NK-cell cytolytic activity. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Oct 2009],
| Size | 50μL/100μL |
| Target | CD244 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 42kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC: 1:100-1:300; ELISA 1:10000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from the Internal region of human CD244. AA range:101-150 |
| Specificity | CD244 Polyclonal Antibody detects endogenous levels of CD244 protein. |
| Gene Name | CD244 |
| Protein Name | Natural killer cell receptor 2B4 |
| Other Name | CD244; / 2B4; / Natural killer cell receptor 2B4; / NK cell activation-inducing ligand; / NAIL; / NK cell type I receptor protein 2B4; / NKR2B4; / h2B4; / CD244 |
| Species | Human |
| Gene ID | 51744 |
| Uniprot | Q9BZW8, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | Q07763 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Membrane; Single-pass type I membrane protein . Cell membrane . Receptor engagement results in a recruitment to lipid drafts essential for the subsequent tyrosine phosphorylation of the ITSMs. . |
| Tissue Expression | Expressed in spleen, PBL, followed by lung, liver, testis and small intestine. Expressed in all natural killer (NK) cells, monocytes and basophils, TCR-gamma/delta+ T-cells, monocytes, basophils, and on a subset of CD8(+) T-cells. |
| Signaling_pathway | Organismal Systems >> Immune system >> Natural killer cell mediated cytotoxicity |
| Research Areas | >>Natural killer cell mediated cytotoxicity |
| Function | Function:Modulate other receptor-ligand interactions to enhance leukocyte activation.,PTM:Phosphorylated.,similarity:Contains 2 Ig-like (immunoglobulin-like) domains.,subunit:Interacts with CD48. Following phosphorylation, it is able to recruit PTPN11/SHP-2 and SH2D1A/SAP. Binding of SH2D1A/SAP to CD244 prevents its association with PTPN11/SHP-2.,tissue specificity:Expressed in spleen, PBL, followed by lung, liver, testis and small intestine. Expressed not only in NK cells, but also on monocytes and basophils., |
