SKU: WM1738 Categories: ,

CD43 [ABT027] mouse mAb

Price range: $268.00 through $328.00

SKU: WM1738-50
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Product details

Background:
The protein encoded by this gene is a major sialoglycoprotein found on the surface of thymocytes, T lymphocytes, monocytes, granulocytes, and some B lymphocytes. It may be part of a physiologic ligand-receptor complex involved in T-cell activation. During T-cell activation, this protein is actively removed from the T-cell-APC (antigen-presenting cell) contact site, suggesting a negative regulatory role in adaptive immune response. [provided by RefSeq, Sep 2011],

Specifications

Size50μL/100μL
TargetCD43
ReactivityHuman
ApplicationsIHC, ELISA
MW(Calculated)40kD
MW(Observed)90kD
Host SpeciesMouse
IsotypeIgG2b,Kappa
Conjugate/ModificationUnmodified
Recommended Dilution RatioIHC 1:200-400; ELISA 1:500-5000 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
FormulationPBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
PurificationThe antibody was affinity-purified from ascites by affinity-chromatography using specific immunogen.
Storage-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
ClonalityMonoclonal
Clone NumberRMM027
ImmunogenSynthesized peptide derived from human CD43 AA range: 300-400
SpecificityThe antibody can specifically recognize human CD43 protein.
Gene NameSPN CD43
Protein NameLeukosialin (GPL115) (Galactoglycoprotein) (GALGP) (Leukocyte sialoglycoprotein) (Sialophorin) (CD antigen CD43) [Cleaved into: CD43 cytoplasmic tail (CD43-ct) (CD43ct)]
Other NameLeukosialin; / Galactoglycoprotein; / GALGP; / Leukocyte sialoglycoprotein; / Sialophorin; / CD antigen CD43
SpeciesHuman
Gene ID6693
UniprotP16150
Species.1
Gene ID.1
Uniprot.1
Species.2
Gene ID.2
Uniprot.2
Cellular LocalizationMembranous
Tissue ExpressionCell surface of thymocytes, T-lymphocytes, neutrophils, plasma cells and myelomas.
Signaling_pathwayEnvironmental Information Processing >> Signaling molecules and interaction >> Cell adhesion molecules
Research Areas>>Cell adhesion molecules
FunctionDisease:CD43 expression is defective on the T-cells of males with the immunodeficiency Wiskott-Aldrich syndrome. Affected males are susceptible to opportunistic infections and do not respond to polysaccharide antigens, reflecting defects in cytotoxic and helper T-cell functions.,Function:One of the major glycoproteins of thymocytes and T lymphocytes. Plays a role in the physicochemical properties of the T-cell surface and in lectin binding. Presents carbohydrate ligands to selectins. Has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. Is a counter receptor for SN/Siglec-1 (By similarity). During T-cell activation is actively removed from the T-cell-APC (antigen-presenting cell) contact site thus suggesting a negative regulatory role in adaptive immune response.,PTM:Glycosylated; has a high content of sialic acid and O-linked carbohydrate structures.,subunit:Interacts with HIPK2 via the cytoplasmic domain.,tissue specificity:Cell surface of thymocytes, T-lymphocytes, neutrophils, plasma cells and myelomas.,
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