Name: CD45 [ABT-CD45] mouse mAb
SKU: WM1387
Price: 268 USD
Availability: InStock

Product details

Background:
CD45, also known as leukocyte common antigen, is a specific marker of hematopoietic cells. CD45 consists of at least four isoforms produced by alternative messenger RNA splicing. The isoforms share a common large intracellular domain and the extracellular domains are rod shaped, heavily glycosylated. It is expressed in all T cells, B cells and NK cells except plasma cells, monocytes, granulocytes and macrophages, but not in erythrocytes, platelets and non hematopoietic systems, Therefore, it can be used as a good marker to distinguish lymphoma or leukemia from non hematopoietic tumors.

Specifications

Target	CD45
Reactivity	Human
Applications	IHC, WB, IF, ELISA
MW(Calculated)
MW(Observed)	200kD
Host Species	Mouse
Isotype	IgG2b,Kappa
Conjugate/Modification	Unmodified
Modification
Recommended Dilution Ratio	IHC 1:200-1000; WB 1:500-2000; IF 1:100-500; ELISA 1:1000-5000 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#YS0004)
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Source
Purification	Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein G affinity chromatography.
Purity
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	ABT-CD45
Immunogen	Synthesized peptide derived from human CD45 (LCA) AA range: 500-600
Sequence
Specificity	The antibody can specifically recognize human CD45 protein, including CD45RA, CD45RB and CD45RO.
Gene Name	PTPRC CD45
Protein Name	Receptor-type tyrosine-protein phosphatase C (Leukocyte common antigen) (L-CA) (T200) (CD antigen CD45)
Other Name	B220; / CD 45; / CD45; / CD45 antigen; / CD45R; / GP180; / L-CA; / LCA; / Leukocyte common antigen; / loc; / Ly-5; / LY5; / Ly5, homolog of; / Lyt-4; / OTTHUMP00000033813; / OTTHUMP00000033816; / OTTHUMP00000033817; / OTTHUMP00000038574; / Protein tyrosine phosphatase receptor type c polypeptide; / Protein tyrosine phosphatase, receptor type C; / protein tyrosine phosphatase, receptor type, C; / Protein tyrosine phosphatase, receptor type, c polypeptide; / Ptprc; / PTPRC_HUMAN; / Receptor-type tyrosine-protein phosphatase C; / T200; / T200 glycoprotein; / T200 leukocyte common antigen
Species	Human
Gene ID-1	5788
Uniprot	P08575-3
Species.1
Gene ID-2
Uniprot.1
Species.2
Gene ID-3
Uniprot.2
Organism-4
Gene ID-4
SwissProt-4
Background	CD45, also known as leukocyte common antigen, is a specific marker of hematopoietic cells. CD45 consists of at least four isoforms produced by alternative messenger RNA splicing. The isoforms share a common large intracellular domain and the extracellular domains are rod shaped, heavily glycosylated. It is expressed in all T cells, B cells and NK cells except plasma cells, monocytes, granulocytes and macrophages, but not in erythrocytes, platelets and non hematopoietic systems, Therefore, it can be used as a good marker to distinguish lymphoma or leukemia from non hematopoietic tumors.
Cellular Localization	Membranous
Tissue Expression	Isoform 1: Detected in thymocytes. Isoform 2: Detected in thymocytes. Isoform 3: Detected in thymocytes. Isoform 4: Not detected in thymocytes. Isoform 5: Detected in thymocytes. Isoform 6: Not detected in thymocytes. Isoform 7: Detected in thymocytes. Isoform 8: Not detected in thymocytes.
Signaling_pathway	Organismal Systems >> Immune system >> T cell receptor signaling pathway;Organismal Systems >> Immune system >> Fc gamma R-mediated phagocytosis;Human Diseases >> Immune disease >> Primary immunodeficiency;Environmental Information Processing >> Signaling molecules and interaction >> Cell adhesion molecules
Research Areas	>>Cell adhesion molecules; / >>T cell receptor signaling pathway; / >>Fc gamma R-mediated phagocytosis; / >>Salmonella infection; / >>Primary immunodeficiency
Function	Alternative products:At least 8 isoforms are produced,Catalytic activity:Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate.,Disease:Defects in PTPRC are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+)SCID) [MIM:608971]. SCID refers to a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients with SCID present in infancy with recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.,Disease:Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain.,Domain:The first PTPase domain interacts with SKAP1.,Function:Required for T-cell activation through the antigen receptor. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits an dephosphorylates SKAP1 and FYN.,online information:CD45 entry,online information:PTPRC mutation db,PTM:Heavily N- and O-glycosylated.,similarity:Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.,similarity:Contains 2 fibronectin type-III domains.,similarity:Contains 2 tyrosine-protein phosphatase domains.,subunit:Binds GANAB and PRKCSH (By similarity). Interacts with SKAP1.,
RRID

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CD45 [ABT-CD45] mouse mAb

Product details

Specifications

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CD45 [ABT-CD45] mouse mAb

Product details

Specifications

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