Name: Cdc2 Polyclonal Antibody
SKU: WP11528
Price: 268 USD
Availability: InStock

Product details

Background:

cyclin dependent kinase 1(CDK1) Homo sapiens The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009],

Specifications

Target	CDK1/CDC2
Reactivity	Human, Mouse, Rat
Applications	WB, IP, ELISA
MW(Calculated)
MW(Observed)	34kD
Host Species	Rabbit
Isotype	IgG
Conjugate/Modification	Unmodified
Modification
Recommended Dilution Ratio	WB 1:500-1:2000; IP 1:50-200; ELISA 1:10000; Not yet tested in other applications.
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Purity
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration	1 mg/ml
Clonality	Polyclonal
Clone Number
Immunogen	The antiserum was produced against synthesized peptide derived from human CDC2. AA range:131-180
Sequence
Specificity	Cdc2 Polyclonal Antibody detects endogenous levels of Cdc2 protein.
Gene Name	CDK1
Protein Name	Cyclin-dependent kinase 1
Other Name	CDK1; / CDC2; / CDC28A; / CDKN1; / P34CDC2; / Cyclin-dependent kinase 1; / CDK1; / Cell division control protein 2 homolog; / Cell division protein kinase 1; / p34 protein kinase
Species	Human
Gene ID-1	983
Uniprot	P06493,
Species.1	Mouse
Gene ID-2	12534
Uniprot.1	P11440,
Species.2	Rat
Gene ID-3	54237
Uniprot.2	P39951
Organism-4
Gene ID-4
SwissProt-4
Background	cyclin dependent kinase 1(CDK1) Homo sapiens The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009],
Cellular Localization	Nucleus. Cytoplasm. Mitochondrion . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm, cytoskeleton, spindle. Cytoplasmic during the interphase. Colocalizes with SIRT2 on centrosome during prophase and on splindle fibers during metaphase of the mitotic cell cycle. Reversibly translocated from cytoplasm to nucleus when phosphorylated before G2-M transition when associated with cyclin-B1. Accumulates in mitochondria in G2-arrested cells upon DNA-damage.
Tissue Expression	Isoform 2 is found in breast cancer tissues.
Signaling_pathway	Cellular Processes >> Cell growth and death >> Cell cycle;Cellular Processes >> Cell growth and death >> p53 signaling pathway;Cellular Processes >> Cell growth and death >> Cellular senescence;Cellular Processes >> Cellular community - eukaryotes >> Gap junction
Research Areas	>>Cell cycle; / >>Oocyte meiosis; / >>p53 signaling pathway; / >>Cellular senescence; / >>Gap junction; / >>Progesterone-mediated oocyte maturation; / >>Human immunodeficiency virus 1 infection; / >>Viral carcinogenesis
Function	Catalytic activity:ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.,Catalytic activity:ATP + a protein = ADP + a phosphoprotein.,enzyme regulation:Phosphorylation at Thr-14 or Tyr-15 inactivates the enzyme, while phosphorylation at Thr-161 activates it.,Function:Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Forms a stable but non-covalent complex with a regulatory subunit and with a cyclin. Interacts with DLGAP5. Isoform 2 is unable to complex with cyclin B1 and also fails to bind to the CDK inhibitor p21. Interacts with catalytically active CCNB1 and RALBP1 during mitosis to form an endocytotic complex during interphase.,
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Cdc2 Polyclonal Antibody

Product details

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Cdc2 Polyclonal Antibody

Product details

Specifications

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