SKU: WP11584 Categories: ,

CEP350 Polyclonal Antibody

Price range: $268.00 through $328.00

SKU: WP11584-100
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Product details

Background:

centrosomal protein 350(CEP350) Homo sapiens The product of this gene is a large protein with a CAP-Gly domain typically found in cytoskeleton-associated proteins. The encoded protein primarily localizes to the centrosome, a non-membraneous organelle that functions as the major microtubule-organizing center in animal cells. The encoded protein directly interacts with another large centrosomal protein and is required to anchor microtubules at the centrosome. It is also implicated in the regulation of a class of nuclear hormone receptors in the nucleus. Several alternatively spliced transcript variants have been found, but their full-length nature has not been determined. [provided by RefSeq, Jul 2008],

Specifications

Size50μL/100μL
TargetCEP350
ReactivityHuman, Mouse
ApplicationsIHC, IF, ELISA
MW(Calculated)351kD
MW(Observed)
Host SpeciesRabbit
IsotypeIgG
Conjugate/ModificationUnmodified
Recommended Dilution RatioIHC 1:100-1:300; ELISA 1:40000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration1 mg/ml
ClonalityPolyclonal
Clone Number
ImmunogenThe antiserum was produced against synthesized peptide derived from human CEP35. AA range:671-720
SpecificityCEP350 Polyclonal Antibody detects endogenous levels of CEP350 protein.
Gene NameCEP350
Protein NameCentrosome-associated protein 350
Other NameCEP350; / CAP350; / KIAA0480; / GM133; / Centrosome-associated protein 350; / Cep350; / Centrosome-associated protein of 350 kDa
SpeciesHuman
Gene ID9857
UniprotQ5VT06
Species.1
Gene ID.1
Uniprot.1
Species.2
Gene ID.2
Uniprot.2
Cellular LocalizationCytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm, cytoskeleton, spindle . Nucleus . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole . Cytoplasm, cytoskeleton, cilium basal body . Associated with mitotic spindles (PubMed:16314388). Nuclear, in discrete foci. Associated with intermediate filaments (PubMed:15615782). Also present in the pericentrosomal area (PubMed:17878239). Localizes on both mother and daughter centrioles. Localizes to an axial position on the mother centriole (PubMed:28625565). Localizes to the distal end of the centriole on the subdistal appendage region (PubMed:28659385). .
Tissue ExpressionDetected in heart, brain, skeletal muscle, testis, placenta, lung, liver, kidney and pancreas.
Signaling_pathway
Research Areas
FunctionFunction:Required for anchoring microtubules to the centrosomes. Required for the integrity of the microtubule network. Recruits PPARA to discrete subcellular compartments and thereby modulates PPARA activity.,PTM:Phosphorylated during mitosis.,similarity:Contains 1 CAP-Gly domain.,subcellular location:Associated with mitotic spindles. Nuclear, in discrete foci. Associated with intermediate filaments.,subunit:Part of a ternary complex that contains CEP350, FGFR1OP and MAPRE1. Interacts directly with FGFR1OP via its C-terminus. Interacts with NR1H3, PPARA, PPARD and PPARG.,tissue specificity:Detected in heart, brain, skeletal muscle, testis, placenta, lung, liver, kidney and pancreas.,
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