Name: COX-2 Polyclonal Antibody
SKU: WP11768
Price: 268 USD
Availability: InStock

Product details

Background:

Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes the inducible isozyme. It is regulated by specific stimulatory events, suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis. [provided by RefSeq, Feb 2009],

Specifications

Size	50μL/100μL
Target	COX2
Reactivity	Human, Mouse, Rat
Applications	WB, IHC, IF, ELISA
MW(Calculated)
MW(Observed)	70kD
Host Species	Rabbit
Isotype	IgG
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:20000; Not yet tested in other applications. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration	1 mg/ml
Clonality	Polyclonal
Clone Number
Immunogen	The antiserum was produced against synthesized peptide derived from human Cox2. AA range:555-604
Specificity	Cox-2 Polyclonal Antibody detects endogenous levels of Cox-2 protein.
Gene Name	PTGS2
Protein Name	Prostaglandin G/H synthase 2
Other Name	PTGS2; / COX2; / Prostaglandin G/H synthase 2; / Cyclooxygenase-2; / COX-2; / PHS II; / Prostaglandin H2 synthase 2; / PGH synthase 2; / PGHS-2; / Prostaglandin-endoperoxide synthase 2
Species	Human
Gene ID	5743
Uniprot	P35354,
Species.1	Mouse
Gene ID.1
Uniprot.1	Q05769
Species.2
Gene ID.2
Uniprot.2
Cellular Localization	Microsome membrane; Peripheral membrane protein. Endoplasmic reticulum membrane; Peripheral membrane protein. Nucleus inner membrane; Peripheral membrane protein. Nucleus outer membrane; Peripheral membrane protein. Detected on the lumenal side of the endoplasmic reticulum and nuclear envelope. .
Tissue Expression	Endothelial cell,Epidermal keratinocytes in primary culture,Lung,Pe
Signaling_pathway	Organismal Systems >> Immune system >> IL-17 signaling pathway;Organismal Systems >> Endocrine system >> Oxytocin signaling pathway;Human Diseases >> Cancer: overview >> Pathways in cancer;Human Diseases >> Cancer: overview >> MicroRNAs in cancer;Human Diseases >> Cancer: specific types >> Small cell lung cancer;Human Diseases >> Neurodegenerative disease >> Alzheimer disease;Human Diseases >> Neurodegenerative disease >> Pathways of neurodegeneration - multiple diseases;Environmental Information Processing >> Signal transduction >> VEGF signaling pathway;Environmental Information Processing >> Signal transduction >> NF-kappa B signaling pathway;Environmental Information Processing >> Signal transduction >> TNF signaling pathway
Research Areas	>>Arachidonic acid metabolism; / >>Metabolic pathways; / >>NF-kappa B signaling pathway; / >>VEGF signaling pathway; / >>C-type lectin receptor signaling pathway; / >>IL-17 signaling pathway; / >>TNF signaling pathway; / >>Retrograde endocannabinoid signaling; / >>Serotonergic synapse; / >>Ovarian steroidogenesis; / >>Oxytocin signaling pathway; / >>Regulation of lipolysis in adipocytes; / >>Alzheimer disease; / >>Pathways of neurodegeneration - multiple diseases; / >>Leishmaniasis; / >>Human cytomegalovirus infection; / >>Human papillomavirus infection; / >>Kaposi sarcoma-associated herpesvirus infection; / >>Pathways in cancer; / >>Chemical carcinogenesis - DNA adducts; / >>MicroRNAs in cancer; / >>Small cell lung cancer
Function	Catalytic activity:Arachidonate + AH(2) + 2 O(2) = prostaglandin H(2) + A + H(2)O.,cofactor:Binds 1 heme B (iron-protoporphyrin IX) group per subunit.,Disease:Likely to play a role in inflammatory diseases such as rheumatoid arthritis.,Function:May have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity.,induction:By cytokines and mitogens.,miscellaneous:This enzyme acts both as a dioxygenase and as a peroxidase.,miscellaneous:This enzyme is the target of nonsteroidal anti-inflammatory drugs such as aspirin.,pathway:Lipid metabolism; prostaglandin biosynthesis.,similarity:Belongs to the prostaglandin G/H synthase family.,similarity:Contains 1 EGF-like domain.,subunit:Homodimer.,