Price range: $268.00 through $328.00
Background:
This gene encodes one of three subunits which combine to form cleavage stimulation factor (CSTF). CSTF is involved in the polyadenylation and 3'end cleavage of pre-mRNAs. Similar to mammalian G protein beta subunits, this protein contains transducin-like repeats. Several transcript variants with different 5' UTR, but encoding the same protein, have been found for this gene. [provided by RefSeq, Jul 2008],
| Size | 50μL/100μL |
| Target | CstF-50 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 48kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:20000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human CSTF1. AA range:1-50 |
| Specificity | CstF-50 Polyclonal Antibody detects endogenous levels of CstF-50 protein. |
| Gene Name | CSTF1 |
| Protein Name | Cleavage stimulation factor subunit 1 |
| Other Name | CSTF1; / Cleavage stimulation factor subunit 1; / CF-1 50 kDa subunit; / Cleavage stimulation factor 50 kDa subunit; / CSTF 50 kDa subunit; / CstF-50 |
| Species | Human |
| Gene ID | 1477 |
| Uniprot | Q05048, |
| Species.1 | Mouse |
| Gene ID.1 | 67337 |
| Uniprot.1 | Q99LC2, |
| Species.2 | Rat |
| Gene ID.2 | 311670 |
| Uniprot.2 | Q5BJQ6 |
| Cellular Localization | Nucleus . |
| Tissue Expression | Muscle,Testis, |
| Signaling_pathway | |
| Research Areas | >>mRNA surveillance pathway |
| Function | Domain:N-terminus mediates homodimerization.,Domain:The WD6 domain is required for interaction with BARD1. WD domains are responsible for interaction with CSTF3.,Function:One of the multiple factors required for polyadenylation and 3'-end cleavage of mammalian pre-mRNAs. May be responsible for the interaction of CSTF with other factors to form a stable complex on the pre-mRNA.,PTM:The N-terminus is blocked.,similarity:Contains 6 WD repeats.,subunit:Homodimer. The CSTF complex is composed of CSTF1 (50 kDa subunit), CSTF2 (64 kDa subunit) and CSTF3 (77 kDa subunit). Interacts directly with CSTF3. Interacts with BARD1., |
