CYP2E1 Polyclonal Antibody
Ucallm_WP11890-50
Price range: $268.00 through $328.00
Product details
Background:
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is induced by ethanol, the diabetic state, and starvation. The enzyme metabolizes both endogenous substrates, such as ethanol, acetone, and acetal, as well as exogenous substrates including benzene, carbon tetrachloride, ethylene glycol, and nitrosamines which are premutagens found in cigarette smoke. Due to its many substrates, this enzyme may be involved in such varied processes as gluconeogenesis, hepatic cirrhosis, diabetes, and cancer. [provided by RefSeq, Jul 2008],
Specifications
| Size | 50μL/100μL |
| Target | CYP2E1 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 56kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:5000; Not yet tested in other applications. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Cytochrome P450 2E1. AA range:371-420 |
| Specificity | CYP2E1 Polyclonal Antibody detects endogenous levels of CYP2E1 protein. |
| Gene Name | CYP2E1 |
| Protein Name | Cytochrome P450 2E1 |
| Other Name | CYP2E1; / CYP2E; / Cytochrome P450 2E1; / 4-nitrophenol 2-hydroxylase; / CYPIIE1; / Cytochrome P450-J |
| Species | Human |
| Gene ID | 1571 |
| Uniprot | P05181, |
| Species.1 | Mouse |
| Gene ID.1 | 13106 |
| Uniprot.1 | Q05421, |
| Species.2 | Rat |
| Gene ID.2 | 25086 |
| Uniprot.2 | P05182 |
| Cellular Localization | Endoplasmic reticulum membrane; Peripheral membrane protein . Microsome membrane; Peripheral membrane protein . Mitochondrion inner membrane; Peripheral membrane protein . Post-translationally targeted to mitochondria. TOMM70 is required for the translocation across the mitochondrial outer membrane. After translocation into the matrix, associates with the inner membrane as a membrane extrinsic protein. . |
| Tissue Expression | Brain,Liver,Lung,PCR rescued clones, |
| Signaling_pathway | |
| Research Areas | >>Steroid hormone biosynthesis; / >>Arachidonic acid metabolism; / >>Linoleic acid metabolism; / >>Metabolism of xenobiotics by cytochrome P450; / >>Drug metabolism - cytochrome P450; / >>Drug metabolism - other enzymes; / >>Metabolic pathways; / >>Non-alcoholic fatty liver disease; / >>Alcoholic liver disease; / >>Chemical carcinogenesis - DNA adducts; / >>Chemical carcinogenesis - reactive oxygen species |
| Function | Catalytic activity:4-nitrophenol + NADPH + O(2) = 4-nitrocatechol + NADP(+) + H(2)O.,cofactor:Heme group.,Function:Metabolizes several precarcinogens, drugs, and solvents to reactive metabolites. Inactivates a number of drugs and xenobiotics and also bioactivates many xenobiotic substrates to their hepatotoxic or carcinogenic forms.,induction:By ethanol and isoniazid.,online information:CYP2E1 alleles,online information:CYP2E1 entry,similarity:Belongs to the cytochrome P450 family., |
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