Price range: $268.00 through $328.00
Background:
The DYKDDDDK peptide (Flag-tag) is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, and then used to separate recombinant, over expressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
| Target | Flag-tag |
| Reactivity | Species independent |
| Applications | WB, ELISA, IP, IF, CoIP |
| MW(Calculated) | |
| MW(Observed) | |
| Host Species | Mouse |
| Isotype | VH IgG1 and VL Kappa |
| Conjugate/Modification | Unmodified |
| Modification | |
| Recommended Dilution Ratio | WB 1:10000-50000; ELISA 1:20000-50000; IP 1:50-1:200; IF 1:1000 -3000 |
| Formulation | PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol. |
| Source | Mouse VH IgG1 and VL Kappa was expressed from 293F cells |
| Purification | Protein A purified |
| Purity | >95% SDS-PAGE |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | 12M11 |
| Immunogen | Synthetic Peptide of Flag-Tag |
| Sequence | |
| Specificity | This recombinant antibody can highly specifically recognize the Flag tags of C-terminal and N-terminal of recombinant protein. Variable region gene of this antibody comes from the mouse gene library immunized with DDDK peptide. Constant region of this antibody is mouse IgG1 and mouse kappa. |
| Gene Name | Flag tag; Flag-tag,DDDDK TAG, DDDDK-TAG, DYKDDDDK tag,DYKDDDDK-tag |
| Protein Name | |
| Other Name | |
| Species | |
| Gene ID-1 | |
| Uniprot | |
| Species.1 | |
| Gene ID-2 | |
| Uniprot.1 | |
| Species.2 | |
| Gene ID-3 | |
| Uniprot.2 | |
| Organism-4 | |
| Gene ID-4 | |
| SwissProt-4 | |
| Background | The DYKDDDDK peptide (Flag-tag) is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, and then used to separate recombinant, over expressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. |
| Cellular Localization | |
| Tissue Expression | |
| Signaling_pathway | |
| Research Areas | |
| Function | |
| RRID |
