Name: U-Blot® DDX58 Rabbit mAb
SKU: WR4466
Price: 268 USD
Availability: InStock

U-Blot® DDX58 Rabbit mAb

Jurkat cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with primary antibody 1:1000. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.

WR4466 Jurkat cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with primary antibody 1:1000. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.

Price range: $268.00 through $328.00

Size

100μl

50μl

Clear

SKU: WR4466-50

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Product details

Background:DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases which are implicated in a number of cellular processes involving RNA binding and alteration of RNA secondary structure. This gene encodes a protein containing RNA helicase-DEAD box protein motifs and a caspase recruitment domain (CARD). It is involved in viral double-stranded (ds) RNA recognition and the regulation of immune response. [provided by RefSeq, Jul 2008],

Specifications

Size	50μL/100μL
Target	DDX58
Reactivity	Human, Mouse, Rat
Applications	WB, IHC, IF, IP, ELISA
MW(Calculated)	107kD
MW(Observed)	107kD
Host Species	Rabbit
Isotype	IgG,Kappa
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	IHC 1:200-1:1000; WB 1:500-1:2000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Purification	Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	RM0686R
Immunogen	The specific immunogen used to produce this antibody is proprietary information.
Specificity	Endogenous
Gene Name	DDX58
Protein Name	Probable ATP-dependent RNA helicase DDX58 (DEAD box protein 58) (RIG-I-like receptor 1) (RLR-1) (Retinoic acid-inducible gene 1 protein) (RIG-1) (Retinoic acid-inducible gene I protein) (RIG-I)
Other Name
Species	Human
Gene ID	23586
Uniprot	O95786,
Species.1	Mouse
Gene ID.1
Uniprot.1	Q6Q899
Species.2
Gene ID.2
Uniprot.2
Cellular Localization	Cytoplasm
Tissue Expression	Present in vascular smooth cells (at protein level).
Signaling_pathway	Organismal Systems >> Immune system >> RIG-I-like receptor signaling pathway;Organismal Systems >> Immune system >> Cytosolic DNA-sensing pathway;Environmental Information Processing >> Signal transduction >> NF-kappa B signaling pathway
Research Areas	>>NF-kappa B signaling pathway; / >>RIG-I-like receptor signaling pathway; / >>Cytosolic DNA-sensing pathway; / >>Hepatitis C; / >>Hepatitis B; / >>Measles; / >>Influenza A; / >>Herpes simplex virus 1 infection; / >>Epstein-Barr virus infection; / >>Coronavirus disease - COVID-19
Function	Domain:The 2 CARD domains are responsible for interaction with and signaling through MAVS.,Domain:The helicase domain is responsible for dsRNA recognition.,Domain:The repressor domain controls homomultimerization and interaction with MAVS.,Function:Involved in innate immune defense against viruses. Upon interaction with intracellular dsRNA produced during viral replication, triggers a transduction cascade involving MAVS/IPS1, which results in the activation of NF-kappa-B, IRF3 and IRF7 and the induction of the expression of antiviral cytokines such as IFN-beta and RANTES (CCL5). Essential for the production of interferons in response to RNA viruses including paramyxoviruses, influenza viruses, Japanese encephalitis virus and HCV.,induction:By bacterial lipopolysaccharide (LPS) in endothelial cells. By IFN-alpha, -beta and -gamma.,PTM:Isgylated. Conjugated to ubiquitin-like protein ISG15 upon IFN-beta stimulation.,similarity:Belongs to the helicase family.,similarity:Contains 1 helicase ATP-binding domain.,similarity:Contains 1 helicase C-terminal domain.,similarity:Contains 2 CARD domains.,subunit:Monomer; maintained as a monomer in an autoinhibited state. Upon viral dsRNA binding and conformation shift, homomultimerizes and interacts with MAVS. Interacts with DHX58/LGP2, IKBKE, TBK1 and TMEM173/STING.,tissue specificity:Present in vascular smooth cells (at protein level).,