DFFB Polyclonal Antibody
Ucallm_WP16061-50
Price range: $268.00 through $328.00
Product details
Background:
Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene but the biological validity of some of these variants has not been determined. [provided by RefSeq, Sep 2013],
Specifications
| Size | 50μL/100μL |
| Target | DFFB |
| Reactivity | Human, Mouse, Rat |
| Applications | IHC, IF, WB |
| MW(Calculated) | |
| MW(Observed) | 37kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:50-200; WB 1:500-2000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | Synthesized peptide derived from human DFFB |
| Specificity | This antibody detects endogenous levels of human DFFB |
| Gene Name | DFFB CAD DFF2 DFF40 |
| Protein Name | DFFB |
| Other Name | DNA fragmentation factor subunit beta; / Caspase-activated deoxyribonuclease; / CAD; / Caspase-activated DNase; / Caspase-activated nuclease; / CPAN; / DNA fragmentation factor 40 kDa subunit; / DFF-40 |
| Species | Human |
| Gene ID | 1677 |
| Uniprot | O76075 |
| Species.1 | |
| Gene ID.1 | |
| Uniprot.1 | |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Cytoplasm. Nucleus. |
| Tissue Expression | Brain,Fetal brain,Lung,Pancreas, |
| Signaling_pathway | Cellular Processes >> Cell growth and death >> Apoptosis |
| Research Areas | >>Apoptosis |
| Function | enzyme regulation:Inhibited by DFFA (DFF45).,Function:Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology.,similarity:Contains 1 CIDE-N domain.,subunit:Heterodimer of DFFA and DFFB., |
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