Price range: $268.00 through $328.00
Background:
The protein encoded by this gene belongs to the eukaryotic diacylglycerol kinase family. It may attenuate protein kinase C activity by regulating diacylglycerol levels in intracellular signaling cascade and signal transduction. Alternative splicing occurs at this locus and multiple transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Nov 2010],
| Size | 50μL/100μL |
| Target | DGK-ζ |
| Reactivity | Human, Mouse, Rat |
| Applications | IHC, IF, ELISA |
| MW(Calculated) | 124kD |
| MW(Observed) | |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:100-1:300; ELISA 1:20000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human DGKZ. AA range:601-650 |
| Specificity | DGK-ζ Polyclonal Antibody detects endogenous levels of DGK-ζ protein. |
| Gene Name | DGKZ |
| Protein Name | Diacylglycerol kinase zeta |
| Other Name | DGKZ; / DAGK6; / Diacylglycerol kinase zeta; / DAG kinase zeta; / Diglyceride kinase zeta; / DGK-zeta |
| Species | Human |
| Gene ID | 8525 |
| Uniprot | Q13574, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | Q80UP3 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Nucleus . Cytoplasm, cytosol . Cell membrane . Cell projection, lamellipodium . |
| Tissue Expression | Highest levels in brain, and substantial levels in skeletal muscle, heart, and pancreas.; [Isoform 2]: Predominantly expressed in muscle. |
| Signaling_pathway | Environmental Information Processing >> Signal transduction >> Phospholipase D signaling pathway |
| Research Areas | >>Glycerolipid metabolism; / >>Glycerophospholipid metabolism; / >>Metabolic pathways; / >>Phosphatidylinositol signaling system; / >>Phospholipase D signaling pathway; / >>Choline metabolism in cancer |
| Function | Catalytic activity:ATP + 1,2-diacylglycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.,Caution:The sequence shown here is derived from an Ensembl automatic analysis pipeline and should be considered as preliminary data.,Function:Displays a strong preference for 1,2-diacylglycerols over 1,3-diacylglycerols, but lacks substrate specificity among molecular species of long chain diacylglycerols. Isoform 2 but not isoform 1 regulates RASGRP1 activity.,PTM:Phosphorylation of the MARCKS homology domain by PKC reduces nuclear accumulation of DGK-zeta.,similarity:Belongs to the eukaryotic diacylglycerol kinase family.,similarity:Contains 1 DAGKc domain.,similarity:Contains 2 ANK repeats.,similarity:Contains 2 phorbol-ester/DAG-type zinc fingers.,subunit:Interacts with the PDZ domain of the syntrophin SNTG1 and that of SNX27. Isoform 2 forms a signaling complex with RASGRP1 and HRAS.,tissue specificity:Highest levels in brain, and substantial levels in skeletal muscle, heart, and pancreas. Isoform 1 is predominantly expressed in muscle., |
