DPYD Polyclonal Antibody
Ucallm_WP15300-50
Price range: $268.00 through $328.00
Product details
Background:
The protein encoded by this gene is a pyrimidine catabolic enzyme and the initial and rate-limiting factor in the pathway of uracil and thymidine catabolism. Mutations in this gene result in dihydropyrimidine dehydrogenase deficiency, an error in pyrimidine metabolism associated with thymine-uraciluria and an increased risk of toxicity in cancer patients receiving 5-fluorouracil chemotherapy. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009],
Specifications
| Size | 50μL/100μL |
| Target | DPYD |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 120kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC: 1:100-300; ELISA 1:20000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from the Internal region of human DPYD. AA range:351-400 |
| Specificity | DPYD Polyclonal Antibody detects endogenous levels of DPYD protein. |
| Gene Name | DPYD |
| Protein Name | Dihydropyrimidine dehydrogenase [NADP(+)] |
| Other Name | DPYD; / Dihydropyrimidine dehydrogenase [NADP; / +; / ]; / DHPDHase; / DPD; / Dihydrothymine dehydrogenase; / Dihydrouracil dehydrogenase |
| Species | Human |
| Gene ID | 1806 |
| Uniprot | Q12882, |
| Species.1 | Mouse |
| Gene ID.1 | 99586 |
| Uniprot.1 | Q8CHR6, |
| Species.2 | Rat |
| Gene ID.2 | 81656 |
| Uniprot.2 | O89000 |
| Cellular Localization | Cytoplasm. |
| Tissue Expression | Found in most tissues with greatest activity found in liver and peripheral blood mononuclear cells. |
| Signaling_pathway | |
| Research Areas | >>Pyrimidine metabolism; / >>beta-Alanine metabolism; / >>Pantothenate and CoA biosynthesis; / >>Drug metabolism - other enzymes; / >>Metabolic pathways |
| Function | Catalytic activity:5,6-dihydrouracil + NADP(+) = uracil + NADPH.,cofactor:Binds 2 4Fe-4S clusters. Contains approximately 33 iron atoms per molecule.,cofactor:Binds 2 FAD.,cofactor:Binds 2 FMN.,Disease:Defects in DPYD are the cause of dihydropyrimidine dehydrogenase deficiency (DPYD deficiency) [MIM:274270]; also known as hereditary thymine-uraciluria or familial pyrimidinemia. DPYD deficiency is a disease characterized by persistent urinary excretion of excessive amounts of uracil, thymine and 5-hydroxymethyluracil. Patients suffering from this disease show a severe reaction to the anticancer drug 5-fluorouracil. This reaction includes stomatitis, Leukopenia, thrombocytopenia, hair loss, diarrhea, fever, marked weight loss, cerebellar ataxia, and neurologic symptoms, progressing to semicoma.,Function:Involved in pyrimidine base degradation. Catalyzes the reduction of uracil and thymine. Also involved the degradation of the chemotherapeutic drug 5-fluorouracil.,pathway:Amino-acid biosynthesis; beta-alanine biosynthesis.,similarity:Belongs to the dihydropyrimidine dehydrogenase family.,similarity:Contains 3 4Fe-4S ferredoxin-type domains.,subunit:Homodimer.,tissue specificity:Found in most tissues with greatest activity found in liver and peripheral blood mononuclear cells., |
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