Name: U-Blot® DRP1 Rabbit mAb
SKU: WR4030
Price: 268 USD
Availability: InStock

U-Blot® DRP1 Rabbit mAb

WR4030

Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue. 1, primary Antibody was diluted at 1:200(4～C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98～C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).

WR4030 Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue. 1, primary Antibody was diluted at 1:200(4～C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98～C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).

Immunohistochemical analysis of paraffin-embedded rat pancreas tissue. 1, primary Antibody was diluted at 1:200(4～C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98～C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).

WR4030 Immunohistochemical analysis of paraffin-embedded rat pancreas tissue. 1, primary Antibody was diluted at 1:200(4～C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98～C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).

Immunohistochemical analysis of paraffin-embedded human brain tissue. 1, primary Antibody was diluted at 1:200(4～C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98～C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).

WR4030 Immunohistochemical analysis of paraffin-embedded human brain tissue. 1, primary Antibody was diluted at 1:200(4～C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98～C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).

Western blot analysis of lysates from Rat brain cells, using DRP1 Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody (WS0002) was used to detect the antibody.

WR4030 Western blot analysis of lysates from Rat brain cells, using DRP1 Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody (WS0002) was used to detect the antibody.

Price range: $268.00 through $328.00

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50μl

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SKU: WR4030-50

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Product details

Background:This gene encodes a member of the dynamin superfamily of GTPases. The encoded protein mediates mitochondrial and peroxisomal division, and is involved in developmentally regulated apoptosis and programmed necrosis. Dysfunction of this gene is implicated in several neurological disorders, including Alzheimer's disease. Mutations in this gene are associated with the autosomal dominant disorder, encephalopathy, lethal, due to defective mitochondrial and peroxisomal fission (EMPF). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2013],

Specifications

Size	50μL/100μL
Target	DRP1
Reactivity	Human, Mouse, Rat, Bovine
Applications	WB, IHC, IF, IP, ELISA
MW(Calculated)	83kD
MW(Observed)	83kD
Host Species	Rabbit
Isotype	IgG,Kappa
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	IHC 1:200-1000; WB 1:1000-5000; IF 1:200-1000; ELISA 1:5000-20000; IP 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Purification	Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	RM0086R
Immunogen	The specific immunogen used to produce this antibody is proprietary information.
Specificity	Endogenous
Gene Name	DNM1L
Protein Name	Dynamin-1-like protein
Other Name	DNM1L; / DLP1; / DRP1; / Dynamin-1-like protein; / Dnm1p/Vps1p-like protein; / DVLP; / Dynamin family member proline-rich carboxyl-terminal domain less; / Dymple; / Dynamin-like protein; / Dynamin-like protein 4; / Dynamin-like protein IV; / HdynIV; / Dynamin-rela
Species	Human
Gene ID	10059
Uniprot	O00429,
Species.1	Mouse
Gene ID.1	74006
Uniprot.1	Q8K1M6,
Species.2	Rat
Gene ID.2	114114
Uniprot.2	O35303
Cellular Localization	Cytoplasm
Tissue Expression	Ubiquitously expressed with highest levels found in skeletal muscles, heart, kidney and brain. Isoform 1 is brain-specific. Isoform 2 and isoform 3 are predominantly expressed in testis and skeletal muscles respectively. Isoform 4 is weakly expressed in brain, heart and kidney. Isoform 5 is dominantly expressed in liver, heart and kidney. Isoform 6 is expressed in neurons.
Signaling_pathway	Cellular Processes >> Cell growth and death >> Necroptosis;Organismal Systems >> Immune system >> NOD-like receptor signaling pathway;Environmental Information Processing >> Signal transduction >> TNF signaling pathway
Research Areas	>>Necroptosis; / >>NOD-like receptor signaling pathway; / >>TNF signaling pathway
Function	Catalytic activity:GTP + H(2)O = GDP + phosphate.,Function:Functions in mitochondrial and peroxisomal division probably by regulating membrane fission. Enzyme hydrolyzing GTP that oligomerizes to form ring-like structures and is able to remodel membranes. May also play a role on organelles of the secretory pathway.,miscellaneous:Isoform 1 and isoform 2 inhibits peroxisomal division when overexpressed while isoform 3 and isoform 4 have no effect.,PTM:Phosphorylated by GSK3B.,similarity:Belongs to the dynamin family.,similarity:Contains 1 GED domain.,subcellular location:Mainly cytosolic. Also membrane-associated. Localizes to mitochondria at spots of division events. Associated with peroxisomal membranes, it is recruited in part by PEX11B. May also be associated with endoplasmic reticulum tubules and cytoplasmic vesicles and found to be perinuclear.,subunit:Homotetramer; N-terminal part binds to the C-terminal part of another DNM1L. Can self-assemble in multimeric ring-like structures. Interacts with FIS1 (By similarity). Interacts with GSK3B.,tissue specificity:Ubiquitously expressed with highest levels found in skeletal muscles, heart, kidney and brain. Isoform 1 is brain-specific while isoform 3 and isoform 4 are predominantly expressed in testis and skeletal muscles respectively. Isoform 2 is weakly expressed in brain, heart and kidney and isoform 5 is dominantly expressed in liver, heart and kidney.,