Price range: $268.00 through $328.00
Background:
The protein encoded by this gene is a glycosylated membrane protein and a non-specific receptor for several chemokines. The encoded protein is the receptor for the human malarial parasites Plasmodium vivax and Plasmodium knowlesi. Polymorphisms in this gene are the basis of the Duffy blood group system. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],
| Size | 50μL/100μL |
| Target | Duffy |
| Reactivity | Human, Mouse |
| Applications | IF, ELISA |
| MW(Calculated) | 36kD |
| MW(Observed) | |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IF 1:200-1:1000; ELISA 1:40000; Not yet tested in other applications; |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human DARC. AA range:1-50 |
| Specificity | Duffy Polyclonal Antibody detects endogenous levels of Duffy protein. |
| Gene Name | DARC |
| Protein Name | Duffy antigen/chemokine receptor |
| Other Name | DARC; / FY; / GPD; / Duffy antigen/chemokine receptor; / Fy glycoprotein; / GpFy; / Glycoprotein D; / Plasmodium vivax receptor; / CD antigen CD234 |
| Species | Human |
| Gene ID | 2532 |
| Uniprot | Q16570, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | Q9QUI6 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Early endosome. Recycling endosome. Membrane; Multi-pass membrane protein. Predominantly localizes to endocytic vesicles, and upon stimulation by the ligand is internalized via caveolae. Once internalized, the ligand dissociates from the receptor, and is targeted to degradation while the receptor is recycled back to the cell membrane. |
| Tissue Expression | Found in adult kidney, adult spleen, bone marrow and fetal liver. In particular, it is expressed along postcapillary venules throughout the body, except in the adult liver. Erythroid cells and postcapillary venule endothelium are the principle tissues expressing duffy. Fy(-A-B) individuals do not express duffy in the bone marrow, however they do, in postcapillary venule endothelium. |
| Signaling_pathway | |
| Research Areas | >>Malaria |
| Function | Disease:Individuals that do not produce the Duffy antigen (FY(A-B-)) are more resistant to vivax malaria. This allele is found predominantly in population of African origin.,Function:Non-specific receptor for many chemokines such as IL-8, GRO, RANTES, MCP-1 and TARC. It is also the receptor for the human malaria parasites Plasmodium vivax and Plasmodium knowlesi.,online information:Blood group antigen gene mutation database,online information:Duffy antigen entry,polymorphism:DARC is responsible for the Duffy blood group system. The molecular basis of the Fy(A)=Fy1/Fy(B)=Fy2 blood group antigens is a single variation in position 42; Gly-42 corresponds to Fy(A) and Asp-42 to Fy(B).,polymorphism:Genetic variation in DARC is associated with white blood cell count quantitative trait locus type 1 (WBCQ1) [MIM:611862]. Peripheral white blood cell count (WBC) is a common clinical measurement, used to determine evidence of acute infammation or infection. Peripheral WBC is the sum of several cell types including neutrophils and lymphocytes, which are the most common types of WBC, as well as less common cell types such as eosinophils, basophils, and monocytes. Elevated WBC has been associated with risk of coronary heart disease, cancer, and all-cause mortality. White blood cell levels have widespread clinical applications including assessment of patients undergoing chemotherapy and evaluation of infection.,similarity:Belongs to the G-protein coupled receptor Duffy family.,tissue specificity:Found in adult kidney, adult spleen, bone marrow and fetal liver. In particular, it is expressed along postcapillary venules throughout the body, except in the adult liver. Erythroid cells and postcapillary venule endothelium are the principle tissues expressing duffy. Fy(-A-B) individuals do not express duffy in the bone marrow, however they do, in postcapillary venule endothelium., |
