Price range: $268.00 through $328.00
Background:
The protein encoded by this gene belongs to the EGR family of C2H2-type zinc-finger proteins. It is a nuclear protein and functions as a transcriptional regulator. The products of target genes it activates are required for differentitation and mitogenesis. Studies suggest this is a cancer suppressor gene. [provided by RefSeq, Dec 2014],
| Size | 50μL/100μL |
| Target | Egr1/2 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 50kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:20000; Not yet tested in other applications. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human EGR1/2. AA range:371-420 |
| Specificity | Egr1/2 Polyclonal Antibody detects endogenous levels of Egr1/2 protein. |
| Gene Name | EGR1/2 |
| Protein Name | Early growth response protein 1/2 |
| Other Name | EGR1; / KROX24; / ZNF225; / Early growth response protein 1; / EGR-1; / AT225; / Nerve growth factor-induced protein A; / NGFI-A; / Transcription factor ETR103; / Transcription factor Zif268; / Zinc finger protein 225; / Zinc finger protein Krox-24; / EGR2; / KROX20 |
| Species | Human |
| Gene ID | 1958;1959 |
| Uniprot | P18146;P11161, |
| Species.1 | Mouse |
| Gene ID.1 | 13653;13654, |
| Uniprot.1 | |
| Species.2 | Rat |
| Gene ID.2 | 24330;114090 |
| Uniprot.2 | P08154;P51774 |
| Cellular Localization | Nucleus . Cytoplasm . |
| Tissue Expression | Detected in neutrophils (at protein level). |
| Signaling_pathway | Organismal Systems >> Endocrine system >> GnRH signaling pathway;Human Diseases >> Neurodegenerative disease >> Prion disease;Environmental Information Processing >> Signal transduction >> Apelin signaling pathway |
| Research Areas | >>Apelin signaling pathway; / >>GnRH signaling pathway; / >>Parathyroid hormone synthesis, secretion and action; / >>AGE-RAGE signaling pathway in diabetic complications; / >>Prion disease; / >>Human T-cell leukemia virus 1 infection |
| Function | Function:Transcriptional regulator. Recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3'(EGR-site). Activates the transcription of target genes whose products are required for mitogenesis and differentiation.,induction:By growth factors.,similarity:Belongs to the EGR C2H2-type zinc-finger protein family.,similarity:Contains 3 C2H2-type zinc fingers., |
