SKU: WP3311 Categories: ,

ELAV1 Polyclonal Antibody

Price range: $268.00 through $328.00

SKU: WP3311-100
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Product details

Background:

ELAV like RNA binding protein 1(ELAVL1) Homo sapiens The protein encoded by this gene is a member of the ELAVL family of RNA-binding proteins that contain several RNA recognition motifs, and selectively bind AU-rich elements (AREs) found in the 3' untranslated regions of mRNAs. AREs signal degradation of mRNAs as a means to regulate gene expression, thus by binding AREs, the ELAVL family of proteins play a role in stabilizing ARE-containing mRNAs. This gene has been implicated in a variety of biological processes and has been linked to a number of diseases, including cancer. It is highly expressed in many cancers, and could be potentially useful in cancer diagnosis, prognosis, and therapy. [provided by RefSeq, Sep 2012],

Specifications

Size50μL/100μL
TargetELAVL1
ReactivityHuman, Mouse
ApplicationsWB, ELISA
MW(Calculated)
MW(Observed)35kD
Host SpeciesRabbit
IsotypeIgG
Conjugate/ModificationUnmodified
Recommended Dilution RatioWB 1:500-2000; ELISA 1:5000-20000
FormulationLiquid in PBS containing 50% glycerol,0.5% BSA and 0.02% sodium azide.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration1 mg/ml
ClonalityPolyclonal
Clone Number
ImmunogenSynthesized peptide derived from part region of human protein
SpecificityELAV1 Polyclonal Antibody detects endogenous levels of protein.
Gene NameELAVL1 HUR
Protein NameELAV-like protein 1 (Hu-antigen R) (HuR)
Other Name
SpeciesHuman
Gene ID1994
UniprotQ15717,
Species.1Mouse
Gene ID.1
Uniprot.1P70372
Species.2
Gene ID.2
Uniprot.2
Cellular LocalizationCytoplasm . Nucleus . Cytoplasm, Stress granule . Cytoplasm, P-body . Translocates into the cytoplasm following phosphorylation by MAPKAPK2 (PubMed:14517288). Likewise, phosphorylation by PRKCD promotes translocation from the nucleus into the cytoplasm, where it is associated with free and cytoskeleton-bound polysomes (PubMed:18285462).Localizes to the stress granules in the presence of PLEKHN1 (By similarity). .
Tissue ExpressionUbiquitous. Detected in brain, liver, thymus and muscle.
Signaling_pathwayOrganismal Systems >> Immune system >> IL-17 signaling pathway;Environmental Information Processing >> Signal transduction >> AMPK signaling pathway
Research Areas>>AMPK signaling pathway; / >>IL-17 signaling pathway
FunctionFunction:Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, HUR binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA, and AUUUUUA motifs.,PTM:Methylated at Arg-217 by CARM1 in macrophages in response to LPS challenge.,similarity:Belongs to the RRM elav family.,similarity:Contains 3 RRM (RNA recognition motif) domains.,subunit:Interacts with ANP32A.,tissue specificity:Ubiquitous.,
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