Price range: $268.00 through $328.00
Background:
The protein encoded by this gene is an aminopeptidase involved in trimming HLA class I-binding precursors so that they can be presented on MHC class I molecules. The encoded protein acts as a monomer or as a heterodimer with ERAP2. This protein may also be involved in blood pressure regulation by inactivation of angiotensin II. Three transcript variants encoding two different isoforms have been found for this gene.[provided by RefSeq, Oct 2010],
| Size | 50μL/100μL |
| Target | ERAP1 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 107kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:20000; Not yet tested in other applications. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human ARTS-1. AA range:441-490 |
| Specificity | ERAP1 Polyclonal Antibody detects endogenous levels of ERAP1 protein. |
| Gene Name | ERAP1 |
| Protein Name | Endoplasmic reticulum aminopeptidase 1 |
| Other Name | ERAP1; / APPILS; / ARTS1; / KIAA0525; / Endoplasmic reticulum aminopeptidase 1; / ARTS-1; / Adipocyte-derived leucine aminopeptidase; / A-LAP; / Aminopeptidase PILS; / Puromycin-insensitive leucyl-specific aminopeptidase; / PILS-AP; / Type 1 tumor necrosis facto |
| Species | Human |
| Gene ID | 51752 |
| Uniprot | Q9NZ08, |
| Species.1 | Mouse |
| Gene ID.1 | 80898 |
| Uniprot.1 | Q9EQH2, |
| Species.2 | Rat |
| Gene ID.2 | 80897 |
| Uniprot.2 | Q9JJ22 |
| Cellular Localization | Endoplasmic reticulum membrane; Single-pass type II membrane protein . |
| Tissue Expression | Ubiquitous. |
| Signaling_pathway | |
| Research Areas | |
| Function | Catalytic activity:Release of an N-terminal amino acid, Xaa-|-Xbb-, in which Xaa is preferably Leu, but may be other amino acids including Met, Cys and Phe.,Caution:It is uncertain whether Met-1 or Met-13 is the initiator.,cofactor:Binds 1 zinc ion per subunit.,Function:Aminopeptidase that plays a central role in peptide trimming, a step required for the generation of most HLA class I-binding peptides. Peptide trimming is essential to customize longer precursor peptides to fit them to the correct length required for presentation on MHC class I molecules. Strongly prefers substrates 9-16 residues long. Rapidly degrades 13-mer to a 9-mer and then stops. Preferentially hydrolyzes the residue Leu and peptides with a hydrophobic C-terminus, while it has weak activity toward peptides with charged C-terminus. May play a role in the inactivation of peptide hormones. May be involved in the regulation of blood pressure through the inactivation of angiotensin II and/or the generation of bradykinin in the kidney.,induction:By IFN-gamma.,PTM:N-glycosylated.,similarity:Belongs to the peptidase M1 family.,subunit:Monomer. May also exist as a heterodimer; with ERAP2.,tissue specificity:Ubiquitous., |
