U-Blot® Ferritin Heavy Chain Rabbit mAb
WR4452 Hela cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with primary antibody 1:1000. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.
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Product details
Background:This gene encodes the heavy subunit of ferritin, the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in ferritin proteins are associated with several neurodegenerative diseases. This gene has multiple pseudogenes. Several alternatively spliced transcript variants have been observed, but their biological validity has not been determined. [provided by RefSeq, Jul 2008],
Specifications
| Size | 50μL/100μL |
| Target | Ferritin heavy chain |
| Reactivity | Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | 21kD |
| MW(Observed) | 21kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM0381R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | FTH1 |
| Protein Name | Ferritin heavy chain |
| Other Name | FTH1; / FTH; / FTHL6; / OK/SW-cl.84; / PIG15; / Ferritin heavy chain; / Ferritin H subunit; / Cell proliferation-inducing gene 15 protein |
| Species | Human |
| Gene ID | 10398 |
| Uniprot | P02794, |
| Species.1 | Mouse |
| Gene ID.1 | 14319 |
| Uniprot.1 | P09528, |
| Species.2 | Rat |
| Gene ID.2 | 296313 |
| Uniprot.2 | Q64122 |
| Cellular Localization | Cytoplasm |
| Tissue Expression | Smooth muscle tissues and in some, but not all, nonmuscle cells. |
| Signaling_pathway | Cellular Processes >> Cellular community - eukaryotes >> Focal adhesion;Cellular Processes >> Cellular community - eukaryotes >> Adherens junction;Cellular Processes >> Cellular community - eukaryotes >> Tight junction;Cellular Processes >> Cell motility >> Regulation of actin cytoskeleton;Organismal Systems >> Immune system >> Leukocyte transendothelial migration;Organismal Systems >> Endocrine system >> Oxytocin signaling pathway;Organismal Systems >> Circulatory system >> Vascular smooth muscle contraction;Environmental Information Processing >> Signal transduction >> cAMP signaling pathway;Environmental Information Processing >> Signal transduction >> cGMP-PKG signaling pathway |
| Research Areas | >>cGMP-PKG signaling pathway; / >>cAMP signaling pathway; / >>Vascular smooth muscle contraction; / >>Axon guidance; / >>Focal adhesion; / >>Tight junction; / >>Leukocyte transendothelial migration; / >>Regulation of actin cytoskeleton; / >>Oxytocin signaling pathway; / >>Shigellosis; / >>Salmonella infection |
| Function | Catalytic activity:4 Fe(2+) + 4 H(+) + O(2) = 4 Fe(3+) + 2 H(2)O.,Function:Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis.,Function:Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney.,miscellaneous:In human liver the heavy chain is the major chain.,online information:Ferritin entry,similarity:Belongs to the ferritin family.,similarity:Contains 1 ferritin-like diiron domain.,subunit:Oligomer of 24 subunits. There are two types of subunits: L (light) chain and H (heavy) chain. The major chain can be light or heavy, depending on the species and tissue type. The functional molecule forms a roughly spherical shell with a diameter of 12 nm and contains a central cavity into which the insoluble mineral iron core is deposited., |
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