Price range: $268.00 through $328.00
Background:
This gene is a member of the protein-tyrosine kinase oncogene family. It encodes a membrane-associated tyrosine kinase that has been implicated in the control of cell growth. The protein associates with the p85 subunit of phosphatidylinositol 3-kinase and interacts with the fyn-binding protein. Alternatively spliced transcript variants encoding distinct isoforms exist. [provided by RefSeq, Jul 2008],
| Size | 50μL/100μL |
| Target | Fyn |
| Reactivity | Human |
| Applications | WB, IHC, IF, FC, ELISA |
| MW(Calculated) | 61kD |
| MW(Observed) | |
| Host Species | Mouse |
| Isotype | |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:200-1:1000; IF 1:200-1:1000; Flow Cyt 1:200-1:400; ELISA 1:10000; Not yet tested in other applications. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | Affinity purification |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | 13A6 |
| Immunogen | Purified recombinant fragment of human Fyn expressed in E. Coli. |
| Specificity | Fyn Monoclonal Antibody detects endogenous levels of Fyn protein. |
| Gene Name | FYN |
| Protein Name | Tyrosine-protein kinase Fyn |
| Other Name | FYN; / Tyrosine-protein kinase Fyn; / Proto-oncogene Syn; / Proto-oncogene c-Fyn; / Src-like kinase; / SLK; / p59-Fyn |
| Species | Human |
| Gene ID | 2534 |
| Uniprot | P06241, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | P39688 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Cytoplasm. Nucleus. Cell membrane. Present and active in lipid rafts. Palmitoylation is crucial for proper trafficking. |
| Tissue Expression | Isoform 1 is highly expressed in the brain. Isoform 2 is expressed in cells of hemopoietic lineages, especially T-lymphocytes. |
| Signaling_pathway | Cellular Processes >> Cellular community - eukaryotes >> Focal adhesion;Cellular Processes >> Cellular community - eukaryotes >> Adherens junction;Organismal Systems >> Immune system >> Platelet activation;Organismal Systems >> Immune system >> Natural killer cell mediated cytotoxicity;Organismal Systems >> Immune system >> T cell receptor signaling pathway;Organismal Systems >> Immune system >> Fc epsilon RI signaling pathway;Organismal Systems >> Nervous system >> Cholinergic synapse;Organismal Systems >> Development and regeneration >> Osteoclast differentiation;Human Diseases >> Neurodegenerative disease >> Prion disease;Environmental Information Processing >> Signal transduction >> Phospholipase D signaling pathway;Environmental Information Processing >> Signal transduction >> Sphingolipid signaling pathway |
| Research Areas | >>Sphingolipid signaling pathway; / >>Phospholipase D signaling pathway; / >>Axon guidance; / >>Osteoclast differentiation; / >>Focal adhesion; / >>Adherens junction; / >>Platelet activation; / >>Natural killer cell mediated cytotoxicity; / >>T cell receptor signaling pathway; / >>Fc epsilon RI signaling pathway; / >>Cholinergic synapse; / >>Prion disease; / >>Pathogenic Escherichia coli infection; / >>Viral myocarditis |
| Function | Catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,cofactor:Manganese.,enzyme regulation:Inhibited by phosphorylation of Tyr-531 by leukocyte common antigen and activated by dephosphorylation of this site.,Function:Implicated in the control of cell growth. Plays a role in the regulation of intracellular calcium levels, with isoform 2 showing the greater ability to mobilize cytoplasmic calcium in comparison to isoform 1. Required in brain development and mature brain function with important roles in the regulation of axon growth, axon guidance, and neurite extension. Blocks axon outgrowth and attraction induced by NTN1 by phosphorylating its receptor DDC.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 SH2 domain.,similarity:Contains 1 SH3 domain.,subcellular location:Present and active in lipid rafts. Present in cell body and along the process of mature and developing oligodendroyctes.,subunit:Associates through its SH3 domain, to the p85 subunit of phosphatidylinositol 3-kinase. Interacts with the FYN-binding protein (FYB). Interacts with phosphorylated TOM1L1. Interacts with CD79A upon activation of the B-cell antigen receptor which increases FYN activity (By similarity). Interacts with PAG1. Interacts (via SH3 domain) with PRMT8. Interacts with SH2D1A and SLAMF1. Interacts (via SH3 domain) with HEV ORF3 protein.,tissue specificity:Isoform 1 is highly expressed in the brain, isoform 2 is expressed in cells of hemopoietic lineages, especially T lymphocytes., |
