SKU: WP12638 Categories: ,

Gα t1 Polyclonal Antibody

Price range: $268.00 through $328.00

SKU: WP12638-100
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Product details

Background:

Transducin is a 3-subunit guanine nucleotide-binding protein (G protein) which stimulates the coupling of rhodopsin and cGMP-phoshodiesterase during visual impulses. The transducin alpha subunits in rods and cones are encoded by separate genes. This gene encodes the alpha subunit in rods. This gene is also expressed in other cells, and has been implicated in bitter taste transduction in rat taste cells. Mutations in this gene result in autosomal dominant congenital stationary night blindness. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Feb 2009],

Specifications

TargetGα t1
ReactivityHuman, Mouse, Rat
ApplicationsWB, ELISA
MW(Calculated)
MW(Observed)36kD
Host SpeciesRabbit
IsotypeIgG
Conjugate/ModificationUnmodified
Modification
Recommended Dilution RatioWB 1:500-1:2000; ELISA 1:40000; Not yet tested in other applications.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Purity
Storage-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration1 mg/ml
ClonalityPolyclonal
Clone Number
ImmunogenThe antiserum was produced against synthesized peptide derived from human GNAT1. AA range:71-120
Sequence
SpecificityGα t1 Polyclonal Antibody detects endogenous levels of Gα t1 protein.
Gene NameGNAT1
Protein NameGuanine nucleotide-binding protein G(t) subunit alpha-1
Other NameGNAT1; / GNATR; / Guanine nucleotide-binding protein G; / t; / subunit alpha-1; / Transducin alpha-1 chain
SpeciesHuman
Gene ID-12779
UniprotP11488,
Species.1Mouse
Gene ID-214685
Uniprot.1P20612
Species.2
Gene ID-3
Uniprot.2
Organism-4
Gene ID-4
SwissProt-4
BackgroundTransducin is a 3-subunit guanine nucleotide-binding protein (G protein) which stimulates the coupling of rhodopsin and cGMP-phoshodiesterase during visual impulses. The transducin alpha subunits in rods and cones are encoded by separate genes. This gene encodes the alpha subunit in rods. This gene is also expressed in other cells, and has been implicated in bitter taste transduction in rat taste cells. Mutations in this gene result in autosomal dominant congenital stationary night blindness. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Feb 2009],
Cellular LocalizationCell projection, cilium, photoreceptor outer segment . Membrane; Peripheral membrane protein . Photoreceptor inner segment . Localizes mainly in the outer segment in the dark-adapted state, whereas is translocated to the inner part of the photoreceptors in the light-adapted state. During dark-adapted conditions, in the presence of UNC119 mislocalizes from the outer segment to the inner part of rod photoreceptors which leads to decreased photoreceptor damage caused by light. .
Tissue ExpressionRod photoreceptor cells (PubMed:1614872). Predominantly expressed in the retina followed by the ciliary body, iris and retinal pigment epithelium (PubMed:22190596).
Signaling_pathway
Research Areas>>Phototransduction
FunctionDisease:Defects in GNAT1 are the cause of congenital stationary night blindness autosomal dominant type 3 (CSNBAD3) [MIM:610444]; also known as congenital stationary night blindness Nougaret type. Congenital stationary night blindness is a non-progressive retinal disorder characterized by impaired night vision.,Function:Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Transducin is an amplifier and one of the transducers of a visual impulse that performs the coupling between rhodopsin and cGMP-phosphodiesterase.,online information:Retina International's Scientific Newsletter,similarity:Belongs to the G-alpha family. G(i/o/t/z) subfamily.,subunit:G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site.,tissue specificity:Rod.,
RRID
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