SKU: WM1314 Categories: ,

G3BP1 [PTR1324] mouse mAb

Price range: $268.00 through $328.00

SKU: WM1314-50
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Product details

Background:
G3BP stress granule assembly factor 1(G3BP1) Homo sapiens This gene encodes one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2008],

Specifications

Size50μL/100μL
TargetG3BP1
ReactivityHuman, Mouse, Rat
ApplicationsWB, IF, ELISA
MW(Calculated)52kD
MW(Observed)68kD
Host SpeciesMouse
IsotypeIgG2b,Kappa
Conjugate/ModificationUnmodified
Recommended Dilution RatioWB 1:500-2000; IF 1:100-500; ELISA 1:1000-5000
FormulationPBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
PurificationRecombinant Antibody expressed in animal component-free (ACF) media, purified via Protein G affinity chromatography.
Storage-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration1 mg/ml
ClonalityMonoclonal
Clone NumberRMMR1324
ImmunogenSynthesized peptide derived from human protein. AA range: 200-300
SpecificityThis antibody detects endogenous levels of G3BP1 protein.
Gene NameG3BP1 G3BP
Protein NameRas GTPase-activating protein-binding protein 1 (G3BP-1) (ATP-dependent DNA helicase VIII) (hDH VIII) (GAP SH3 domain-binding protein 1)
Other NameRas GTPase-activating protein-binding protein 1; / G3BP-1; / EC 3.6.4.13; / ATP-dependent DNA helicase VIII; / hDH VIII; / GAP SH3 domain-binding protein 1
SpeciesHuman
Gene ID10146
UniprotQ13283,
Species.1Mouse
Gene ID.127041
Uniprot.1P97855
Species.2
Gene ID.2
Uniprot.2
Cellular Localization
Tissue ExpressionUbiquitous.
Signaling_pathwayOrganismal Systems >> Immune system >> Cytosolic DNA-sensing pathway
Research Areas
FunctionATP- and magnesium-dependent helicase that plays an essential role in innate immunity . Participates in the DNA-triggered cGAS/STING pathway by promoting the DNA binding and activation of CGAS. Enhances also DDX58-induced type I interferon production probably by helping DDX58 at sensing pathogenic RNA . In addition, plays an essential role in stress granule formation . Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends . Unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency . Acts unidirectionally by moving in the 5' to 3' direction along the bound single-stranded DNA . Phosphorylation-dependent sequence-specific endoribonuclease in vitro . Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR .
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