Price range: $268.00 through $328.00
Background:
Soluble guanylate cyclases are heterodimeric proteins that catalyze the conversion of GTP to 3', 5'-cyclic GMP and pyrophosphate. The protein encoded by this gene is an alpha subunit of this complex and it interacts with a beta subunit to form the guanylate cyclase enzyme, which is activated by nitric oxide. Several transcript variants encoding a few different isoforms have been found for this gene. [provided by RefSeq, Jan 2012],
| Size | 50μL/100μL |
| Target | GCS-α-1 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, CoIP, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 77kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:40000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human GCS-alpha-1. AA range:374-423 |
| Specificity | GCS-α-1 Polyclonal Antibody detects endogenous levels of GCS-α-1 protein. |
| Gene Name | GUCY1A3 |
| Protein Name | Guanylate cyclase soluble subunit alpha-3 |
| Other Name | GUCY1A3; / GUC1A3; / GUCSA3; / GUCY1A1; / Guanylate cyclase soluble subunit alpha-3; / GCS-alpha-3; / GCS-alpha-1; / Soluble guanylate cyclase large subunit |
| Species | Human |
| Gene ID | 2982 |
| Uniprot | Q02108, |
| Species.1 | Mouse |
| Gene ID.1 | 60596 |
| Uniprot.1 | Q9ERL9, |
| Species.2 | Rat |
| Gene ID.2 | |
| Uniprot.2 | P19686 |
| Cellular Localization | Cytoplasm . |
| Tissue Expression | Detected in brain cortex and lung (at protein level). |
| Signaling_pathway | Cellular Processes >> Cellular community - eukaryotes >> Gap junction;Organismal Systems >> Immune system >> Platelet activation;Organismal Systems >> Endocrine system >> Oxytocin signaling pathway;Organismal Systems >> Circulatory system >> Vascular smooth muscle contraction;Environmental Information Processing >> Signal transduction >> cGMP-PKG signaling pathway |
| Research Areas | >>Purine metabolism; / >>Metabolic pathways; / >>cGMP-PKG signaling pathway; / >>Vascular smooth muscle contraction; / >>Gap junction; / >>Platelet activation; / >>Circadian entrainment; / >>Long-term depression; / >>Oxytocin signaling pathway; / >>Renin secretion; / >>Salivary secretion |
| Function | Catalytic activity:GTP = 3',5'-cyclic GMP + diphosphate.,enzyme regulation:Activated by nitric oxide in the presence of magnesium or manganese ions.,miscellaneous:There are two types of guanylate cyclases: soluble forms and membrane-associated receptor forms.,similarity:Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.,similarity:Contains 1 guanylate cyclase domain.,subunit:Heterodimer of an alpha and a beta chain., |
