Price range: $268.00 through $328.00
Background:
catalytic activity:ATP + glycerol = ADP + sn-glycerol 3-phosphate., function:Key enzyme in the regulation of glycerol uptake and metabolism., pathway:Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1., similarity:Belongs to the FGGY kinase family., subcellular location:In sperm the majority of the enzyme is bound to mitochondria.,
| Size | 50μL/100μL |
| Target | GK2 |
| Reactivity | Human, Mouse |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 61kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:20000; Not yet tested in other applications. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human GK2. AA range:281-330 |
| Specificity | GK2 Polyclonal Antibody detects endogenous levels of GK2 protein. |
| Gene Name | GK2 |
| Protein Name | Glycerol kinase 2 |
| Other Name | GK2; / GKP2; / GKTA; / Glycerol kinase 2; / GK 2; / Glycerokinase 2; / ATP:glycerol 3-phosphotransferase 2; / Glycerol kinase; / testis specific 2 |
| Species | Human |
| Gene ID | 2712 |
| Uniprot | Q14410, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | Q9WU65 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Mitochondrion outer membrane; Single-pass type IV membrane protein . Cytoplasm . In sperm the majority of the enzyme is bound to mitochondria. . |
| Tissue Expression | Testis-specific (PubMed:33536340). Expressed in the midpiece of spermatozoa (PubMed:28852571). |
| Signaling_pathway | Organismal Systems >> Endocrine system >> PPAR signaling pathway |
| Research Areas | >>Glycerolipid metabolism; / >>Metabolic pathways; / >>PPAR signaling pathway |
| Function | Catalytic activity:ATP + glycerol = ADP + sn-glycerol 3-phosphate.,Function:Key enzyme in the regulation of glycerol uptake and metabolism.,pathway:Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1.,similarity:Belongs to the FGGY kinase family.,subcellular location:In sperm the majority of the enzyme is bound to mitochondria., |
