SKU: WM1130 Categories: ,

HAO1 Monoclonal Antibody [Mix]

Price range: $268.00 through $328.00

SKU: WM1130-50
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Product details

Background:
This gene is one of three related genes that have 2-hydroxyacid oxidase activity yet differ in encoded protein amino acid sequence, tissue expression and substrate preference. Subcellular location of the encoded protein is the peroxisome. Specifically, this gene is expressed primarily in liver and pancreas and the encoded protein is most active on glycolate, a two-carbon substrate. The protein is also active on 2-hydroxy fatty acids. The transcript detected at high levels in pancreas may represent an alternatively spliced form or the use of a multiple near-consensus upstream polyadenylation site. [provided by RefSeq, Jul 2008],

Specifications

Size50μL/100μL
TargetHAO1
ReactivityMouse, Rat
ApplicationsWB, IHC, IF
MW(Calculated)
MW(Observed)41kD
Host SpeciesMouse
IsotypeIgG2b,kappa
Conjugate/ModificationUnmodified
Recommended Dilution RatioWB 1:1000-2000; IF 1:200; IHC 1:50-300 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
FormulationPBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
PurificationThe antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Storage-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
ClonalityMonoclonal
Clone Number7G12
ImmunogenRecombinant Protein of HAO1,AA range 35-85
SpecificityThe antibody detects endogenous HAO1 protein.
Gene NameHAO1
Protein NameHydroxyacid oxidase 1
Other NameHydroxyacid oxidase 1; / HAOX1; / Glycolate oxidase; / GOX
SpeciesHuman
Gene ID54363
UniprotQ9UJM8,
Species.1Mouse
Gene ID.1
Uniprot.1Q9WU19
Species.2
Gene ID.2
Uniprot.2
Cellular LocalizationPeroxisome matrix .
Tissue ExpressionHighly expressed in liver.
Signaling_pathwayCellular Processes >> Transport and catabolism >> Peroxisome
Research Areas>>Glyoxylate and dicarboxylate metabolism; / >>Metabolic pathways; / >>Carbon metabolism; / >>Peroxisome
FunctionCatalytic activity:(S)-2-hydroxy acid + O(2) = 2-oxo acid + H(2)O(2).,cofactor:FMN.,Function:Has 2-hydroxyacid oxidase activity. Most active on the 2-carbon substrate glycolate, but is also active on 2-hydroxy fatty acids.,pathway:Organic acid metabolism; glycolic acid degradation; 3-phospho-D-glyceric acid from glycolic acid: step 1/4.,similarity:Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.,similarity:Contains 1 FMN hydroxy acid dehydrogenase domain.,tissue specificity:Liver.,
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