U-Blot® HDAC6 Rabbit mAb
WR4549 K562 cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with primary antibody 1:1000. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.
Price range: $268.00 through $328.00
Product details
Background:Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It contains an internal duplication of two catalytic domains which appear to function independently of each other. This protein possesses histone deacetylase activity and represses transcription. [provided by RefSeq, Jul 2008],
Specifications
| Size | 50μL/100μL |
| Target | HDAC6 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, IP, ELISA |
| MW(Calculated) | 131kD |
| MW(Observed) | 160kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:2000-1:10000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM0826R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | HDAC6 |
| Protein Name | Histone deacetylase 6 |
| Other Name | HDAC6; / KIAA0901; / JM21; / Histone deacetylase 6; / HD6 |
| Species | Human |
| Gene ID | 10013 |
| Uniprot | Q9UBN7, |
| Species.1 | Mouse |
| Gene ID.1 | 15185 |
| Uniprot.1 | Q9Z2V5 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Cytoplasm . Cytoplasm, cytoskeleton . Nucleus . Perikaryon . Cell projection, dendrite . Cell projection, axon . It is mainly cytoplasmic, where it is associated with microtubules. . |
| Tissue Expression | Brain,Epithelium,Kidney,Muscle,Ovary,Placenta, |
| Signaling_pathway | Organismal Systems >> Immune system >> Neutrophil extracellular trap formation;Human Diseases >> Neurodegenerative disease >> Amyotrophic lateral sclerosis |
| Research Areas | >>Neutrophil extracellular trap formation; / >>Amyotrophic lateral sclerosis; / >>Alcoholism; / >>Viral carcinogenesis |
| Function | Catalytic activity:Hydrolysis of an N(6)-acetyl-lysine residue of a histone to yield a deacetylated histone.,Function:Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes (By similarity). Plays a central role in microtubule-dependent cell motility via deacetylation of tubulin.,PTM:Sumoylated in vitro.,PTM:Ubiquitinated. Its polyubiquitination however does not lead to its degradation.,similarity:Belongs to the histone deacetylase family. Type 2 subfamily.,similarity:Contains 1 UBP-type zinc finger.,subcellular location:It is mainly cytoplasmic, where it is associated with microtubules.,subunit:Interacts with CBFA2T3, HDAC11 and SIRT2. Interacts with F-actin. Interacts with BBIP10., |
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