Name: hHR23b mouse mAb
SKU: WM0697
Price: 268 USD
Availability: InStock

Product details

Background:
The protein encoded by this gene is one of two human homologs of Saccharomyces cerevisiae Rad23, a protein involved in the nucleotide excision repair (NER). This protein was found to be a component of the protein complex that specifically complements the NER defect of xeroderma pigmentosum group C (XP-c) cell extracts in vitro. This protein was also shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, and thus this protein may be involved in the ubiquitin mediated proteolytic pathway in cells. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Sep 2011],

Specifications

Size	50μL/100μL
Target	Rad23B
Reactivity	Human, Mouse, Rat, Monkey, Hamster
Applications	WB, ICC, IHC
MW(Calculated)
MW(Observed)	58kD
Host Species	Mouse
Isotype
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	WB 1:1000; ICC 1:100
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification	The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	14D6
Immunogen	Purified recombinant human hHR23b protein fragments expressed in E.coli.
Specificity	This antibody detects endogenous levels of hHR23b and does not cross-react with related proteins.
Gene Name	hr23b
Protein Name
Other Name	hHR 23b; / hHR23B; / HR 23B; / HR23B; / mHR 23B; / mHR23B; / P58; / RAD 23B; / RAD23; / S. cerevisiae; / homolog B; / RAD23 homolog B; / S. cerevisiae; / RAD23 homolog B; / RAD23 yeast homolog of B; / RAD23B; / RD23B_HUMAN; / UV excision repair protein RAD23 homolog B; / XP C repair complementing complex 58 kDa; / XP C repair complementing complex 58 kDa protein; / XP C repair complementing protein; / XP-C repair-complementing complex 58 kDa protein; / XPC repair complementing complex 58 kDa; / XPC repair complementing complex 58 kDa protein; / XPC repair complementing protein.
Species	Human
Gene ID	5887
Uniprot	P54727,
Species.1	Mouse
Gene ID.1
Uniprot.1	P54728
Species.2
Gene ID.2
Uniprot.2
Cellular Localization	Nucleus. Cytoplasm. The intracellular distribution is cell cycle dependent. Localized to the nucleus and the cytoplasm during G1 phase. Nuclear levels decrease during S-phase; upon entering mitosis, relocalizes in the cytoplasm without association with chromatin.
Tissue Expression	Epithelium,Testis,Thymus,Uterus,
Signaling_pathway	Genetic Information Processing >> Folding, sorting and degradation >> Protein processing in endoplasmic reticulum
Research Areas	>>Nucleotide excision repair; / >>Protein processing in endoplasmic reticulum
Function	Domain:The ubiquitin-like domain mediates interaction with MJD.,Function:Plays a central role both in proteosomal degradation of misfolded proteins and DNA repair. Central component of a complex required to couple deglycosylation and proteasome-mediated degradation of misfolded proteins in the endoplasmic reticulun that are retrotranslocated in the cytosol. Involved in DNA excision repair by stabilizing XPC protein. May play a part in DNA damage recognition and/or in altering chromatin structure to allow access by damage-processing enzymes.,similarity:Belongs to the RAD23 family.,similarity:Contains 1 STI1 domain.,similarity:Contains 1 ubiquitin-like domain.,similarity:Contains 2 UBA domains.,subunit:Component of a complex required to couple retrotranslocation, ubiquitination and deglycosylation composed of NGLY1, SAKS1, AMFR, VCP and RAD23B (By similarity). Interacts with the 26S proteasome. Interacts directly with NGLY1. Heterodimer of a 125 kDa subunit (p125) and of a 58 kDa subunit (p58). Interacts with MJD and XPC.,