Name: U-Blot® Histone 1.0 Rabbit pAb
SKU: WP12685
Price: 268 USD
Availability: InStock

Product details

Background:

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-independent histone that is a member of the histone H1 family. [provided by RefSeq, Oct 2015],

Specifications

Target	Histone H1.0
Reactivity	Human, Mouse, Rat
Applications	WB, IHC, IF, ELISA
MW(Calculated)
MW(Observed)	28kD
Host Species	Rabbit
Isotype	IgG
Conjugate/Modification	Unmodified
Modification
Recommended Dilution Ratio	WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:40000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#YS0004)
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Purity
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration	1 mg/ml
Clonality	Polyclonal
Clone Number
Immunogen	The antiserum was produced against synthesized peptide derived from human Histone 1F0. AA range:71-120
Sequence
Specificity	Histone 1.0 Polyclonal Antibody detects endogenous levels of Histone 1.0 protein.
Gene Name	H1F0
Protein Name	Histone H1.0
Other Name	H1F0; / H1FV; / Histone H1.0; / Histone H1'; / Histone H1; / 0
Species	Human
Gene ID-1	3005
Uniprot	P07305,
Species.1	Mouse
Gene ID-2
Uniprot.1	P10922
Species.2
Gene ID-3
Uniprot.2
Organism-4
Gene ID-4
SwissProt-4
Background	Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-independent histone that is a member of the histone H1 family. [provided by RefSeq, Oct 2015],
Cellular Localization	Nucleus . Chromosome . The RNA edited version has been localized to nuclear speckles. During mitosis, it appears in the vicinity of condensed chromosomes.
Tissue Expression	Colon,Epithelium,Placenta,
Signaling_pathway
Research Areas
Function	Function:Histones H1 are necessary for the condensation of nucleosome chains into higher order structures. The H1F0 histones are found in cells that are in terminal stages of differentiation or that have low rates of cell division.,induction:Both the unedited and the RNA edited versions are induced by butyrate (at protein level). Only RNA edited version is induced by DTT, vinblastine or TNF (at protein level).,online information:Histone H1 entry,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylated on Ser-17 in RNA edited version.,RNA editing:Partially edited. In approximately 3.6% of the mRNA molecules, a new initiator methionine is created by a single uridine insertion in the 5'-UTR, causing an N-terminal extension of 99 amino acids. The existence of the RNA edited version is supported by direct protein sequencing by MS/MS of the following peptides specific to that version: 12-21; 22-33; 37-47; 48-67; 68-83; 84-94 and 97-113. The RNA edited version is called ET-H1.0.,similarity:Belongs to the histone H1/H5 family.,subcellular location:The RNA edited version has been localized to nuclear speckles. During mitosis, it appears in the vicinity of condensed chromosomes.,
RRID

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U-Blot® Histone 1.0 Rabbit pAb

Product details

Specifications

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U-Blot® Histone 1.0 Rabbit pAb

Product details

Specifications

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