U-Blot® Histone H2A.X Rabbit pAb

Price range: $268.00 through $328.00

SKU: WP12692-100
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Product details

Background:Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq, Oct 2015],

Specifications

TargetHistone H2A.X
ReactivityHuman,Mouse,Rat
ApplicationWB,IHC,IF,CoIP,ELISA
MW(Calculated)
MW(Observed)19kD
Host SpeciesRabbit
IsotypeIgG
ConjugateUnconjugated
ModificationUnmodified
Modification site
Recommended dilution ratioWB 1:500-1:2000;IHC 1:100-1:300;IF 1:200-1:1000;IP 1:200-500;ELISA 1:10000;Not yet tested in other applications.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Purity
Storage-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration1 mg/ml
ClonalityPolyclonal
Clone Number
ImmunogenThe antiserum was produced against synthesized peptide derived from human Histone H2A.X. AA range:94-143
Sequence
SpecificityHistone H2A.X Polyclonal Antibody detects endogenous levels of Histone H2A.X protein.
Gene nameH2AFX
Protein nameHistone H2A.x
Alternative nameH2AFX;H2AX;Histone H2A.x;H2a/x
Organism-1Human
Gene ID‑13014
SwissProt-1P16104
Organism-2Mouse
Gene ID‑215270
SwissProt-2P27661
Organism-3
Gene ID‑3
SwissProt-3
Organism-4
Gene ID‑4
SwissProt-4
Cellular LocalizationNucleus . Chromosome .
Unnamed: 38
Unnamed: 39
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