Specifications
| Size | 50μL/100μL |
| Target | HMGCR |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, IP, ELISA |
| MW(Calculated) | 98kD |
| MW(Observed) | 98kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:100-1:500; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM1309R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | HMGCR |
| Protein Name | 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMG-CoA reductase) (EC 1.1.1.34) |
| Other Name | |
| Species | Human |
| Gene ID | 3156 |
| Uniprot | P04035, |
| Species.1 | Mouse |
| Gene ID.1 | 15357 |
| Uniprot.1 | Q01237, |
| Species.2 | Rat |
| Gene ID.2 | 25675 |
| Uniprot.2 | P51639 |
| Cellular Localization | Endoplasmic reticulum membrane; Multi-pass membrane protein . Peroxisome membrane; Multi-pass membrane protein . |
| Tissue Expression | [Isoform 1]: Ubiquitously expressed with the highest levels in the cerebellum, fetal brain, testis, skin and adrenal gland.; [Isoform 2]: Detected in the cerebellum, fetal brain, testis and adrenal gland.; [Isoform 3]: Low abundance except in skin, esophagus, and uterine cervix. |
| Signaling_pathway | Environmental Information Processing >> Signal transduction >> AMPK signaling pathway |
| Research Areas | |
| Function | Catalyzes the conversion of (3S)-hydroxy-3-methylglutaryl-CoA (HMG-CoA) to mevalonic acid, the rate-limiting step in the synthesis of cholesterol and other isoprenoids, thus plays a critical role in cellular cholesterol homeostasis . HMGCR is the main target of statins, a class of cholesterol-lowering drugs . |
