HSC 70 Polyclonal Antibody
Ucallm_WP5850-50
Price range: $268.00 through $328.00
Product details
Background:
This gene encodes a member of the heat shock protein 70 family, which contains both heat-inducible and constitutively expressed members. This protein belongs to the latter group, which are also referred to as heat-shock cognate proteins. It functions as a chaperone, and binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011],
Specifications
| Size | 50μL/100μL |
| Target | HSC70 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF |
| MW(Calculated) | |
| MW(Observed) | 70-72kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC:1:50-300; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | Synthesized peptide derived from the Internal region of human HSC 70. AA range: 588-638 |
| Specificity | The antibody detects endogenous HSC 70 protein. |
| Gene Name | HSPA8 HSC70 HSP73 HSPA10 |
| Protein Name | Heat shock cognate 71 kDa protein |
| Other Name | HSPA8; / HSC70; / HSP73; / HSPA10; / Heat shock cognate 71 kDa protein; / Heat shock 70 kDa protein 8 |
| Species | Human |
| Gene ID | 3312 |
| Uniprot | P11142, |
| Species.1 | Mouse |
| Gene ID.1 | 15481 |
| Uniprot.1 | P63017, |
| Species.2 | Rat |
| Gene ID.2 | 24468 |
| Uniprot.2 | P63018 |
| Cellular Localization | Cytoplasm. Melanosome. Nucleus, nucleolus. Cell membrane. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Translocates rapidly from the cytoplasm to the nuclei, and especially to the nucleoli, upon heat shock. |
| Tissue Expression | Ubiquitous. |
| Signaling_pathway | Organismal Systems >> Immune system >> Antigen processing and presentation;Organismal Systems >> Endocrine system >> Estrogen signaling pathway;Organismal Systems >> Aging >> Longevity regulating pathway - multiple species;Human Diseases >> Neurodegenerative disease >> Prion disease;Environmental Information Processing >> Signal transduction >> MAPK signaling pathway;Genetic Information Processing >> Folding, sorting and degradation >> Protein processing in endoplasmic reticulum |
| Research Areas | >>Spliceosome; / >>MAPK signaling pathway; / >>Protein processing in endoplasmic reticulum; / >>Endocytosis; / >>Longevity regulating pathway - multiple species; / >>Antigen processing and presentation; / >>Estrogen signaling pathway; / >>Prion disease; / >>Legionellosis; / >>Toxoplasmosis; / >>Measles; / >>Lipid and atherosclerosis |
| Function | Function:Chaperone. Isoform 2 may function as an endogenous inhibitory regulator of HSC70 by competing the co-chaperones.,induction:Constitutively synthesized.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the heat shock protein 70 family.,subcellular location:Translocates rapidly from the cytoplasm to the nuclei, and especially to the nucleoli, upon heat shock. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.,subunit:Interacts with HSPH1/HSP105. Interacts with IRAK1BP1 (By similarity). Interacts with PACRG and TSC2. Interacts with SV40 VP1.,tissue specificity:Ubiquitous., |
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