Price range: $268.00 through $328.00
Background:This gene encodes a nuclear protein containing a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2009],
| Size | 50μL/100μL |
| Target | LSD1 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, IP, ELISA |
| MW(Calculated) | 93kD |
| MW(Observed) | 110kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM0084R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | KDM1A AOF2 KDM1 KIAA0601 LSD1 |
| Protein Name | LSD1 |
| Other Name | KDM1A; / AOF2; / KDM1; / KIAA0601; / LSD1; / Lysine-specific histone demethylase 1A; / BRAF35-HDAC complex protein BHC110; / Flavin-containing amine oxidase domain-containing protein 2 |
| Species | Human |
| Gene ID | 6773 |
| Uniprot | O60341, |
| Species.1 | Mouse |
| Gene ID.1 | 99982 |
| Uniprot.1 | Q6ZQ88 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Nucleus |
| Tissue Expression | Human small intestine,Lung, |
| Signaling_pathway | Cellular Processes >> Cell growth and death >> Necroptosis;Organismal Systems >> Immune system >> Toll-like receptor signaling pathway;Organismal Systems >> Immune system >> NOD-like receptor signaling pathway;Organismal Systems >> Immune system >> Chemokine signaling pathway;Organismal Systems >> Development and regeneration >> Osteoclast differentiation;Human Diseases >> Cancer: overview >> Pathways in cancer;Environmental Information Processing >> Signal transduction >> JAK-STAT signaling pathway |
| Research Areas | >>Chemokine signaling pathway; / >>Necroptosis; / >>Osteoclast differentiation; / >>NOD-like receptor signaling pathway; / >>C-type lectin receptor signaling pathway; / >>JAK-STAT signaling pathway; / >>Hepatitis C; / >>Hepatitis B; / >>Measles; / >>Influenza A; / >>Human papillomavirus infection; / >>Kaposi sarcoma-associated herpesvirus infection; / >>Herpes simplex virus 1 infection; / >>Epstein-Barr virus infection; / >>Coronavirus disease - COVID-19; / >>Pathways in cancer |
| Function | cofactor:FAD.,Domain:The SWIRN domain may act as an anchor site for a histone tail.,Function:Histone demethylase that demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Demethylates both mono- and di-methylated 'Lys-4' of histone H3. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3 'Lys-4' on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. May also demethylate 'Lys-9' of histone H3, a specific tag for epigenetic transcriptional repression, thereby leading to derepression of androgen receptor target genes. Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Demethylates and stabilizes the DNA methylase DNMT1. Required for gastrulation during embryogenesis.,similarity:Belongs to the flavin monoamine oxidase family.,similarity:Contains 1 SWIRM domain.,subunit:Component of a BHC histone deacetylase complex that contains HDAC1, HDAC2, HMG20B/BRAF35, KDM1, RCOR1/CoREST and PHF21A/BHC80. The BHC complex may also contain ZMYM2, ZNF217, ZMYM3, GSE1 and GTF2I. In the complex, RCOR1/CoREST strongly enhances the demethylase activity and protects it from the proteasome while PHF21A/BHC80 inhibits the demethylase activity. Interacts with the androgen receptor (AR).,tissue specificity:Ubiquitously expressed., |
