SKU: WP12934 Categories: ,

KLF8 Polyclonal Antibody

Price range: $268.00 through $328.00

SKU: WP12934-100
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Product details

Background:

This gene encodes a protein which is a member of the Sp/KLF family of transcription factors. Members of this family contain a C-terminal DNA-binding domain with three Kruppel-like zinc fingers. The encoded protein is thought to play an important role in the regulation of epithelial to mesenchymal transition, a process which occurs normally during development but also during metastasis. A pseudogene has been identified on chromosome 16. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2009],

Specifications

Size50μL/100μL
TargetKLF8
ReactivityHuman, Mouse
ApplicationsIHC, IF, ELISA
MW(Calculated)39kD
MW(Observed)
Host SpeciesRabbit
IsotypeIgG
Conjugate/ModificationUnmodified
Recommended Dilution RatioIHC 1:100-1:300; ELISA 1:10000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration1 mg/ml
ClonalityPolyclonal
Clone Number
ImmunogenThe antiserum was produced against synthesized peptide derived from human KLF8. AA range:191-240
SpecificityKLF8 Polyclonal Antibody detects endogenous levels of KLF8 protein.
Gene NameKLF8
Protein NameKrueppel-like factor 8
Other NameKLF8; / BKLF3; / ZNF741; / Krueppel-like factor 8; / Basic krueppel-like factor 3; / Zinc finger protein 741
SpeciesHuman
Gene ID11279
UniprotO95600,
Species.1Mouse
Gene ID.1
Uniprot.1Q8BLM0
Species.2
Gene ID.2
Uniprot.2
Cellular LocalizationNucleus .
Tissue ExpressionUbiquitous.
Signaling_pathway
Research Areas
FunctionFunction:Transcriptional repressor and activator. Binds to CACCC-boxes promoter elements. Also binds the GT-box of cyclin D1 promoter and mediates cell cycle progression at G(1) phase as a downstream target of focal adhesion kinase (FAK).,PTM:Sumoylation at Lys-67 represses transcriptional activity and reduces cell cycle progression into the G(1) phase. Has no effect on subcellular location.,similarity:Belongs to the Sp1 C2H2-type zinc-finger protein family.,similarity:Contains 3 C2H2-type zinc fingers.,subunit:Interacts with corepressor CtBP2. Interacts with PIAS1, PIAS2, AND PIAS4; the interaction with each ligase sumoylates KLF8.,tissue specificity:Ubiquitous.,
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