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Home/Reagents/Antibody/Primary antibody/Monoclonal Antibody/Mouse mAb/KLHL13 Monoclonal Antibody
SKU: WM0283 Categories: ,

KLHL13 Monoclonal Antibody

Ucallm_WM0283-50

Price range: $268.00 through $328.00

SKU: WM0283-50
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Product details

Background:
This gene encodes a BTB and kelch domain containing protein and belongs to the kelch repeat domain containing superfamily of proteins. The encoded protein functions as an adaptor protein that complexes with Cullin 3 and other proteins to form the Cullin 3-based E3 ubiquitin-protein ligase complex. This complex is necessary for proper chromosome segregation and completion of cytokinesis. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010],

Specifications

Size50μL/100μL
TargetKLHL13
ReactivityHuman
ApplicationsWB, IHC, IF, FC, ELISA
MW(Calculated)74kD
MW(Observed)
Host SpeciesMouse
Isotype
Conjugate/ModificationUnmodified
Recommended Dilution RatioWB 1:500-1:2000; IHC 1:200-1:1000; IF 1:200-1:1000; Flow Cyt 1:200-1:400; ELISA 1:10000; Not yet tested in other applications. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
PurificationAffinity purification
Storage-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
ClonalityMonoclonal
Clone Number14A2
ImmunogenPurified recombinant fragment of human KLHL13 expressed in E. Coli.
SpecificityKLHL13 Monoclonal Antibody detects endogenous levels of KLHL13 protein.
Gene NameKLHL13
Protein NameKelch-like protein 13
Other NameKLHL13; / BKLHD2; / KIAA1309; / Kelch-like protein 13; / BTB and kelch domain-containing protein 2
SpeciesHuman
Gene ID90293
UniprotQ9P2N7,
Species.1Mouse
Gene ID.1
Uniprot.1Q80TF4
Species.2
Gene ID.2
Uniprot.2
Cellular Localizationmidbody,Cul3-RING ubiquitin ligase complex,
Tissue ExpressionBrain,Testis,Thalamus,Whole embryo,
Signaling_pathway
Research Areas>>Ubiquitin mediated proteolysis
FunctionFunction:Substrate-specific adapter for a CUL3-based E3 ubiquitin-protein ligase complex. Within this complex, controls the dynamic behavior of AURKB on mitotic chromosomes and thereby coordinates faithful mitotic progression and completion of cytokinesis.,pathway:Protein modification; protein ubiquitination.,similarity:Contains 1 BACK (BTB/Kelch associated) domain.,similarity:Contains 1 BTB (POZ) domain.,similarity:Contains 6 Kelch repeats.,subunit:Forms a complex with CUL3 and KLHL9. Interacts with AURKB. Interacts with CUL3.,
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