Name: Laminin γ-3 Polyclonal Antibody
SKU: WP12973
Price: 268 USD
Availability: InStock

Product details

Background:

Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biol

Specifications

Size	50μL/100μL
Target	Laminin γ-3
Reactivity	Human, Mouse
Applications	WB, IHC, IF, ELISA
MW(Calculated)
MW(Observed)	170kD
Host Species	Rabbit
Isotype	IgG
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:40000; Not yet tested in other applications. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration	1 mg/ml
Clonality	Polyclonal
Clone Number
Immunogen	The antiserum was produced against synthesized peptide derived from human LAMC3. AA range:1361-1410
Specificity	Laminin γ-3 Polyclonal Antibody detects endogenous levels of Laminin γ-3 protein.
Gene Name	LAMC3
Protein Name	Laminin subunit gamma-3
Other Name	LAMC3; / Laminin subunit gamma-3; / Laminin-12 subunit gamma; / Laminin-14 subunit gamma; / Laminin-15 subunit gamma
Species	Human
Gene ID	10319
Uniprot	Q9Y6N6,
Species.1	Mouse
Gene ID.1	23928
Uniprot.1	Q9R0B6
Species.2
Gene ID.2
Uniprot.2
Cellular Localization	Secreted, extracellular space, extracellular matrix, basement membrane.
Tissue Expression	Broadly expressed in: skin, heart, lung, and the reproductive tracts.
Signaling_pathway	Cellular Processes >> Cellular community - eukaryotes >> Focal adhesion;Human Diseases >> Cancer: overview >> Pathways in cancer;Human Diseases >> Cancer: specific types >> Small cell lung cancer;Environmental Information Processing >> Signal transduction >> PI3K-Akt signaling pathway;Environmental Information Processing >> Signaling molecules and interaction >> ECM-receptor interaction
Research Areas	>>PI3K-Akt signaling pathway; / >>Focal adhesion; / >>ECM-receptor interaction; / >>Toxoplasmosis; / >>Amoebiasis; / >>Human papillomavirus infection; / >>Pathways in cancer; / >>Small cell lung cancer
Function	Domain:Domain IV is globular.,Domain:The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.,Function:Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.,similarity:Contains 1 laminin IV type A domain.,similarity:Contains 1 laminin N-terminal domain.,similarity:Contains 11 laminin EGF-like domains.,subunit:Laminin is a complex glycoprotein, consisting of three different polypeptide chains (alpha, beta, gamma), which are bound to each other by disulfide bonds into a cross-shaped molecule comprising one long and three short arms with globules at each end. Gamma-3 is a subunit of laminin-12.,tissue specificity:Broadly expressed in: skin, heart, lung, and the reproductive tracts.,