LMP7A Monoclonal Antibody
Ucallm_WM0301-50
Price range: $268.00 through $328.00
Product details
Background:
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 3 (proteasome beta 5 subunit) in the immu
Specifications
| Size | 50μL/100μL |
| Target | LMP7A |
| Reactivity | Human, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | 30kD |
| MW(Observed) | |
| Host Species | Mouse |
| Isotype | |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:200-1:1000; IF 1:200-1:1000; ELISA 1:10000; Not yet tested in other applications. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | Affinity purification |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | 19H2 |
| Immunogen | Purified recombinant fragment of human LMP7A expressed in E. Coli. |
| Specificity | LMP7A Monoclonal Antibody detects endogenous levels of LMP7A protein. |
| Gene Name | PSMB8 |
| Protein Name | Proteasome subunit beta type-8 |
| Other Name | PSMB8; / LMP7; / PSMB5i; / RING10; / Y2; / Proteasome subunit beta type-8; / Low molecular mass protein 7; / Macropain subunit C13; / Multicatalytic endopeptidase complex subunit C13; / Proteasome component C13; / Proteasome subunit beta-5i; / Really interesting |
| Species | Human |
| Gene ID | 5696 |
| Uniprot | P28062, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | P28063, |
| Species.2 | Rat |
| Gene ID.2 | 24968 |
| Uniprot.2 | P28064 |
| Cellular Localization | Cytoplasm . Nucleus . |
| Tissue Expression | Blood,Skin, |
| Signaling_pathway | Genetic Information Processing >> Folding, sorting and degradation >> Proteasome |
| Research Areas | >>Proteasome |
| Function | Alternative products:Additional isoforms seem to exist,Catalytic activity:Cleavage of peptide bonds with very broad specificity.,Function:The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides.,similarity:Belongs to the peptidase T1B family.,subunit:The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel. This subunit is part of the immunoproteasome where it displaces the equivalent housekeeping subunit PSMB5. Directly interacts with POMP., |
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