SKU: WP13045 Categories: ,

mAChR M4 Polyclonal Antibody

Price range: $268.00 through $328.00

SKU: WP13045-100
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Product details

Background:

The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The clinical implications of this receptor are unknown; however, mouse studies link its function to adenylyl cyclase inhibition. [provided by RefSeq, Jul 2008],

Specifications

Size50μL/100μL
TargetmAChR M4
ReactivityHuman, Mouse, Rat
ApplicationsWB, IF, ELISA
MW(Calculated)
MW(Observed)53kD
Host SpeciesRabbit
IsotypeIgG
Conjugate/ModificationUnmodified
Recommended Dilution RatioWB 1:500-1:2000; IF 1:200-1:1000; ELISA 1:10000; Not yet tested in other applications.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration1 mg/ml
ClonalityPolyclonal
Clone Number
ImmunogenThe antiserum was produced against synthesized peptide derived from human CHRM4. AA range:236-285
SpecificitymAChR M4 Polyclonal Antibody detects endogenous levels of mAChR M4 protein.
Gene NameCHRM4
Protein NameMuscarinic acetylcholine receptor M4
Other NameCHRM4; / Muscarinic acetylcholine receptor M4
SpeciesHuman
Gene ID1132
UniprotP08173,
Species.1Mouse
Gene ID.112672
Uniprot.1P32211,
Species.2Rat
Gene ID.225111
Uniprot.2P08485
Cellular LocalizationCell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein.
Tissue ExpressionBrain,
Signaling_pathwayCellular Processes >> Cell motility >> Regulation of actin cytoskeleton;Organismal Systems >> Nervous system >> Cholinergic synapse
Research Areas>>Neuroactive ligand-receptor interaction; / >>Cholinergic synapse; / >>Regulation of actin cytoskeleton
FunctionFunction:The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is inhibition of adenylate cyclase.,similarity:Belongs to the G-protein coupled receptor 1 family.,
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