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MAD1 Polyclonal Antibody
Ucallm_WP13049-50
Price range: $268.00 through $328.00
Product details
Background:
MAD1L1 is a component of the mitotic spindle-assembly checkpoint that prevents the onset of anaphase until all chromosome are properly aligned at the metaphase plate. MAD1L1 functions as a homodimer and interacts with MAD2L1. MAD1L1 may play a role in cell cycle control and tumor suppression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015],
Specifications
| Size | 50μL/100μL |
| Target | MAD1 |
| Reactivity | Human, Mouse |
| Applications | IF, ELISA |
| MW(Calculated) | 83kD |
| MW(Observed) | |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IF 1:200-1:1000; ELISA 1:10000; Not yet tested in other applications; |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human MAD1. AA range:394-443 |
| Specificity | MAD1 Polyclonal Antibody detects endogenous levels of MAD1 protein. |
| Gene Name | MAD1L1 |
| Protein Name | Mitotic spindle assembly checkpoint protein MAD1 |
| Other Name | MAD1L1; / MAD1; / TXBP181; / Mitotic spindle assembly checkpoint protein MAD1; / Mitotic arrest deficient 1-like protein 1; / MAD1-like protein 1; / Mitotic checkpoint MAD1 protein homolog; / HsMAD1; / hMAD1; / Tax-binding protein 181 |
| Species | Human |
| Gene ID | 8379 |
| Uniprot | Q9Y6D9, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | Q9WTX8 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Nucleus . Chromosome, centromere, kinetochore . Nucleus envelope . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm, cytoskeleton, spindle . Cytoplasm, cytoskeleton, spindle pole . Co-localizes with TPR at the nucleus envelope during interphase and throughout the cell cycle (PubMed:22351768, PubMed:18981471). From the beginning to the end of mitosis, it is seen to move from a diffusely nuclear distribution to the centrosome, to the spindle midzone and finally to the midbody (PubMed:9546394). Localizes to kinetochores during prometaphase (PubMed:22351768, PubMed:29162720). Does not localize to kinetochores during metaphase (PubMed:29162720). Colocalizes with NEK2 at the kinetochore (PubMed:14978040). Colocalizes with IK at spindle poles during metaphase and anaphase (PubMed:22351768). .; [Isoform 3]: Cytoplasm . |
| Tissue Expression | [Isoform 1]: Expressed in hepatocellular carcinomas and hepatoma cell lines (at protein level).; [Isoform 3]: Expressed in hepatocellular carcinomas and hepatoma cell lines (at protein level). |
| Signaling_pathway | Cellular Processes >> Cell growth and death >> Cell cycle |
| Research Areas | >>Cell cycle; / >>Oocyte meiosis; / >>Progesterone-mediated oocyte maturation; / >>Human T-cell leukemia virus 1 infection; / >>Viral carcinogenesis |
| Function | Disease:Defects in MAD1L1 are involved in the development and/or progression of various types of cancer.,Function:Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. May recruit MAD2L1 to unattached kinetochores. Has a role in the correct positioning of the septum. Required for anchoring MAD2L1 to the nuclear periphery.,induction:Increased by TP53.,PTM:Phosphorylated; by BUB1. Become hyperphosphorylated in late S through M phases or after mitotic spindle damage. Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the MAD1 family.,subcellular location:From the beginning to the end of mitosis, it is seen to move from a diffusely nuclear distribution to the centrosome, to the spindle midzone and finally to the midbody.,subunit:Homodimer. Heterodimerizes with MAD2L1 in order to form a tetrameric MAD1L1-MAD2L1 core complex. Perturbation of the original MAD1L1-MAD2L1 structure by the spindle checkpoint may decrease MAD2L1 affinity for MAD1L1. CDC20 can compete with MAD1L1 for MAD2L1 binding, until the attachment and/or tension dampen the checkpoint signal, preventing further release of MAD2L1 on to CDC20. Also able to interact with the BUB1/BUB3 complex and the viral Tax protein. Interacts with TPR.,tissue specificity:Expressed weakly at G0/G1 and highly at late S and G2/M phase., |
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