SKU: WR4446 Categories: ,

U-Blot® MAPKAPK-2 Rabbit mAb

Price range: $268.00 through $328.00

SKU: WR4446-50
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Product details

Background:This gene encodes a member of the Ser/Thr protein kinase family. This kinase is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, this kinase is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo. Two transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],

Specifications

Size50μL/100μL
TargetMAPKAPK2
ReactivityHuman, Mouse, Rat
ApplicationsWB, IF, IP, ELISA
MW(Calculated)46kD
MW(Observed)46,55kD
Host SpeciesRabbit
IsotypeIgG,Kappa
Conjugate/ModificationUnmodified
Recommended Dilution RatioWB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200;
FormulationPBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
PurificationRecombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
ClonalityMonoclonal
Clone NumberRM0666R
ImmunogenThe specific immunogen used to produce this antibody is proprietary information.
SpecificityEndogenous
Gene NameMAPKAPK2
Protein NameMAP kinase-activated protein kinase 2
Other NameMAPKAPK2; / MAP kinase-activated protein kinase 2; / MAPK-activated protein kinase 2; / MAPKAP kinase 2; / MAPKAP-K2; / MAPKAPK-2; / MK-2; / MK2
SpeciesHuman
Gene ID9261
UniprotP49137,
Species.1Mouse
Gene ID.117164
Uniprot.1P49138
Species.2
Gene ID.2
Uniprot.2
Cellular LocalizationCytoplasm . Nucleus . Phosphorylation and subsequent activation releases the autoinhibitory helix, resulting in the export from the nucleus into the cytoplasm.
Tissue ExpressionExpressed in all tissues examined.
Signaling_pathwayCellular Processes >> Cell growth and death >> Cellular senescence;Organismal Systems >> Nervous system >> Neurotrophin signaling pathway;Environmental Information Processing >> Signal transduction >> MAPK signaling pathway;Environmental Information Processing >> Signal transduction >> VEGF signaling pathway
Research Areas>>MAPK signaling pathway; / >>Cellular senescence; / >>VEGF signaling pathway; / >>C-type lectin receptor signaling pathway; / >>Neurotrophin signaling pathway; / >>Kaposi sarcoma-associated herpesvirus infection; / >>Viral carcinogenesis
FunctionCatalytic activity:ATP + a protein = ADP + a phosphoprotein.,enzyme regulation:Seems to be activated by two distinct pathways: the first involves the stimulation of p42/p44 MAPK by growth factors, the second, triggered by stress and heat shock, depends on the activation of MPK2 and upstream MAPKK/MAPKKK.,Function:Its physiological substrate seems to be the small heat shock protein (HSP27/HSP25). In vitro can phosphorylate glycogen synthase at 'Ser-7' and tyrosine hydroxylase (on 'Ser-19' and 'Ser-40'). This kinase phosphorylates Ser in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue (By similarity). Mediates both ERK and p38 MAPK/MAPK14 dependent neutrophil responses. Participates in TNF alpha-stimulated exocytosis of secretory vesicles in neutrophils. Plays a role in phagocytosis-induced respiratory burst activity.,PTM:Phosphorylated and activated by MAP kinase.,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.,similarity:Contains 1 protein kinase domain.,subunit:Interacts with PHC2.,tissue specificity:Expressed in all tissues examined.,
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