Price range: $268.00 through $328.00
Background:
This gene encodes an anti-apoptotic protein, which is a member of the Bcl-2 family. Alternative splicing results in multiple transcript variants. The longest gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene products (isoform 2 and isoform 3) promote apoptosis and are death-inducing. [provided by RefSeq, Oct 2010],
| Size | 50μL/100μL |
| Target | Mcl-1 |
| Reactivity | Human |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 40kD in human,39kD in mouse and rat |
| Host Species | Mouse |
| Isotype | |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:200-1:1000; IF 1:200-1:1000; ELISA 1:10000; Not yet tested in other applications. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | Affinity purification |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | 19A5 |
| Immunogen | Purified recombinant fragment of human MCL-1 expressed in E. Coli. |
| Specificity | Mcl-1 Monoclonal Antibody detects endogenous levels of Mcl-1 protein. |
| Gene Name | MCL1 |
| Protein Name | Induced myeloid leukemia cell differentiation protein Mcl-1 |
| Other Name | MCL1; / BCL2L3; / Induced myeloid leukemia cell differentiation protein Mcl-1; / Bcl-2-like protein 3; / Bcl2-L-3; / Bcl-2-related protein EAT/mcl1; / mcl1/EAT |
| Species | Human |
| Gene ID | 4170 |
| Uniprot | Q07820, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | P97287 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Membrane; Single-pass membrane protein . Cytoplasm. Mitochondrion. Nucleus, nucleoplasm. Cytoplasmic, associated with mitochondria. |
| Tissue Expression | Ewing sarcoma,Mammary gland,Myeloid leukemia cell,Neuroblastoma,Placenta,Th |
| Signaling_pathway | Cellular Processes >> Cell growth and death >> Apoptosis;Human Diseases >> Cancer: overview >> MicroRNAs in cancer;Environmental Information Processing >> Signal transduction >> JAK-STAT signaling pathway;Environmental Information Processing >> Signal transduction >> PI3K-Akt signaling pathway |
| Research Areas | >>PI3K-Akt signaling pathway; / >>Apoptosis; / >>JAK-STAT signaling pathway; / >>MicroRNAs in cancer |
| Function | Function:Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 1 inhibits apoptosis while isoform 2 promotes it.,induction:Expression increases early during phorbol-ester induced differentiation along the monocyte/macrophage pathway in myeloid leukemia cell lines ML-1. Rapidly up-regulated by CSF2 in ML-1 cells. Up-regulated by heat-shock induced differentiation. Expression increases early during retinoic acid-induced differentiation.,PTM:Cleaved by CASP3 during apoptosis. In intact cells cleavage occurs preferentially after Asp-127, yielding a pro-apoptotic 28 kDa C-terminal fragment.,PTM:Phosphorylated on Thr-163. Treatment with taxol or okadaic acid induces phosphorylation on additional sites.,PTM:Rapidly degraded in the absence of phosphorylation on Thr-163 in the PEST region.,similarity:Belongs to the Bcl-2 family.,subcellular location:Cytoplasmic, associated with mitochondria.,subunit:Interacts with BAD, BOK, BIK and BFM (By similarity). Interacts with PMAIP1. Isoform 1 interacts with BAX, BAK1, TPT1 and BCL2L11. Heterodimer of isoform 1 and isoform 2. Homodimers of isoform 1 or isoform 2 are not detected. Isoform 2 does not interact with pro-apoptototic BCL2-related proteins., |
