Price range: $268.00 through $328.00
Background:
This protein is a transcription factor that negatively regulates other myogenic family proteins. Studies of the mouse homolog, I-mf, show that it interferes with myogenic factor function by masking nuclear localization signals and preventing DNA binding. Knockout mouse studies show defects in the formation of vertebrae and ribs that also involve cartilage formation in these structures. [provided by RefSeq, Jul 2008],
| Size | 50μL/100μL |
| Target | MDFI |
| Reactivity | Human, Mouse |
| Applications | WB, ELISA |
| MW(Calculated) | |
| MW(Observed) | 25kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; ELISA 1:10000; Not yet tested in other applications. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human MDFI. AA range:103-152 |
| Specificity | MDFI Polyclonal Antibody detects endogenous levels of MDFI protein. |
| Gene Name | MDFI |
| Protein Name | MyoD family inhibitor |
| Other Name | MDFI; / MyoD family inhibitor; / Myogenic repressor I-mf |
| Species | Human |
| Gene ID | 4188 |
| Uniprot | Q99750, |
| Species.1 | Mouse |
| Gene ID.1 | 17240 |
| Uniprot.1 | P70331 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Nucleus . Cytoplasm . |
| Tissue Expression | Fetal brain,Muscle, |
| Signaling_pathway | |
| Research Areas | |
| Function | Function:Inhibits the transactivation activity of the Myod family of myogenic factors and represses myogenesis. Acts by associating with Myod family members and retaining them in the cytoplasm by masking their nuclear localization signals. Can also interfere with the DNA-binding activity of Myod family members. Plays an important role in trophoblast and chondrogenic differentiation. Regulates the transcriptional activity of TCF7L1/TCF3 by interacting directly with TCF7L1/TCF3 and preventing it from binding DNA. Binds to the axin complex, resulting in an increase in the level of free beta-catenin. Affects axin regulation of the WNT and JNK signaling pathways.,similarity:Belongs to the MDFI family.,subunit:The C-terminus interacts with AXIN1 and LEF1., |
