SKU: WP13308 Categories: ,

MMP-16 Polyclonal Antibody

Price range: $268.00 through $328.00

SKU: WP13308-100
Enter the quantity you would like, or drag the panel to find the quantity and price that best for you
Bulk QuoteAuthorize OEM & ODM

Product details

Background:

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The encoded protein activates MMP2 by cleavage. This gene was once referred to as MT-MMP2, but was renamed as MT-MMP3 or MMP16. [provided by RefSeq, Oct 2010],

Specifications

Size50μL/100μL
TargetMMP-16
ReactivityHuman, Mouse, Rat
ApplicationsWB, IHC, IF, ELISA
MW(Calculated)
MW(Observed)70kD
Host SpeciesRabbit
IsotypeIgG
Conjugate/ModificationUnmodified
Recommended Dilution RatioWB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:10000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration1 mg/ml
ClonalityPolyclonal
Clone Number
ImmunogenThe antiserum was produced against synthesized peptide derived from human MMP-16. AA range:551-600
SpecificityMMP-16 Polyclonal Antibody detects endogenous levels of MMP-16 protein.
Gene NameMMP16
Protein NameMatrix metalloproteinase-16
Other NameMMP16; / MMPX2; / Matrix metalloproteinase-16; / MMP-16; / MMP-X2; / Membrane-type matrix metalloproteinase 3; / MT-MMP 3; / MTMMP3; / Membrane-type-3 matrix metalloproteinase; / MT3-MMP; / MT3MMP
SpeciesHuman
Gene ID4325
UniprotP51512,
Species.1Mouse
Gene ID.117389
Uniprot.1Q9WTR0,
Species.2Rat
Gene ID.265205
Uniprot.2O35548
Cellular Localization[Isoform Long]: Cell membrane; Single-pass type I membrane protein; Extracellular side . Localized at the cell surface of melanoma cells.; [Isoform Short]: Secreted, extracellular space, extracellular matrix. Cell surface. Localized at the cell surface of melanoma cells.
Tissue ExpressionExpressed in heart, brain, placenta, ovary and small intestine. Isoform Short is found in the ovary.
Signaling_pathwayHuman Diseases >> Cancer: overview >> MicroRNAs in cancer
Research Areas>>Parathyroid hormone synthesis, secretion and action; / >>MicroRNAs in cancer
Functioncofactor:Binds 1 zinc ion per subunit.,cofactor:Calcium.,developmental stage:Expressed in tissues undergoing reconstruction. Present in fetal tissues, especially in brain. Expression seems to decline with advanced development.,Domain:The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.,enzyme regulation:TIMP-2 shows little inhibitory activity compared to TIMP-1. TIMP-1 seems to have less binding affinity than TIMP-2 for the short isoform.,Function:Endopeptidase that degrades various components of the extracellular matrix, such as collagen type III and fibronectin. Activates progelatinase A. Involved in the matrix remodeling of blood vessels. The short isoform cleaves fibronectin and also collagen type III, but at lower rate. It has no effect on type I, II, IV and V collagen. However, upon interaction with CSPG4, it may be involved in degradation and invasion of type I collagen by melanoma cells.,PTM:The precursor is cleaved by a furin endopeptidase.,similarity:Belongs to the peptidase M10A family.,similarity:Contains 4 hemopexin-like domains.,subcellular location:Localized at the cell surface of melanoma cells.,subunit:Interacts with CSPG4 through CSPG4 chondroitin sulfate glycosaminoglycan.,tissue specificity:Expressed in heart, brain, placenta, ovary and small intestine. The short isoform is found in the ovary.,
Please login to post questions
Back to Top
Product has been added to your cart