Name: U-Blot® MMP3 Rabbit mAb
SKU: WR4147
Price: 268 USD
Availability: InStock

U-Blot® MMP3 Rabbit mAb

Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4°C, over night with a 1:2500 dilution . The Dylight 800-conjugated Goat anti-Rabbit antibody(Cat:WS0446) was used to detect the antibody.

WR4147 Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4°C, over night with a 1:2500 dilution . The Dylight 800-conjugated Goat anti-Rabbit antibody(Cat:WS0446) was used to detect the antibody.

Western blot analysis of lysates from HepG2 cells, using MMP3 Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody (WS0002) was used to detect the antibody.

WR4147 Western blot analysis of lysates from HepG2 cells, using MMP3 Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody (WS0002) was used to detect the antibody.

Immunohistochemical analysis of paraffin-embedded rat liver tissue. 1, primary Antibody was diluted at 1:200(4～C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98～C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).

WR4147 Immunohistochemical analysis of paraffin-embedded rat liver tissue. 1, primary Antibody was diluted at 1:200(4～C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98～C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).

Price range: $268.00 through $328.00

Size

100μl

50μl

Clear

SKU: WR4147-50

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Product details

Background:matrix metallopeptidase 3(MMP3) Homo sapiens Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes an enzyme which degrades fibronectin, laminin, collagens III, IV, IX, and X, and cartilage proteoglycans. The enzyme is thought to be involved in wound repair, progression of atherosclerosis, and tumor initiation. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq, Jul 2008],

Specifications

Size	50μL/100μL
Target	MMP-3
Reactivity	Human, Mouse, Rat, Geese
Applications	WB, IHC, IF, ELISA
MW(Calculated)	54kD
MW(Observed)	54kD
Host Species	Rabbit
Isotype	IgG,Kappa
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	IHC 1:200-1:1000; WB 1:1000-1:5000; IF 1:200-1:1000; ELISA 1:5000-1:20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Purification	Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	RM0292R
Immunogen	The specific immunogen used to produce this antibody is proprietary information.
Specificity	Endogenous
Gene Name	MMP3
Protein Name	Stromelysin-1
Other Name	MMP3; / STMY1; / Stromelysin-1; / SL-1; / Matrix metalloproteinase-3; / MMP-3; / Transin-1
Species	Human
Gene ID	4314
Uniprot	P08254,
Species.1	Mouse
Gene ID.1	17392
Uniprot.1	P28862,
Species.2	Rat
Gene ID.2
Uniprot.2	P03957
Cellular Localization	Cytoplasm
Tissue Expression	Fibroblast,Lung,Synovium,
Signaling_pathway	Organismal Systems >> Immune system >> IL-17 signaling pathway;Human Diseases >> Cancer: overview >> Transcriptional misregulation in cancer;Human Diseases >> Cancer: specific types >> Prostate cancer;Human Diseases >> Immune disease >> Rheumatoid arthritis;Environmental Information Processing >> Signal transduction >> TNF signaling pathway
Research Areas	>>IL-17 signaling pathway; / >>TNF signaling pathway; / >>Coronavirus disease - COVID-19; / >>Transcriptional misregulation in cancer; / >>Prostate cancer; / >>Rheumatoid arthritis; / >>Lipid and atherosclerosis
Function	Catalytic activity:Preferential cleavage where P1', P2' and P3' are hydrophobic residues.,cofactor:Binds 2 zinc ions per subunit.,cofactor:Binds 4 calcium ions per subunit.,Domain:The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.,Function:Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.,similarity:Belongs to the peptidase M10A family.,similarity:Contains 4 hemopexin-like domains.,